Q2SSE6 (IF2_MYCCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2

Gene names

Name:	infB
Ordered Locus Names:	MCAP_0333

Organism

Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]

Taxonomic identifier

340047 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma mycoides group › Mycoplasma capricolum › Mycoplasma capricolum subsp. capricolum

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	Translation initiation factor IF-2 HAMAP-Rule MF_00100		PRO_1000008280
Regions
	Nucleotide binding	128 – 135	8	GTP By similarity
	Nucleotide binding	175 – 179	5	GTP By similarity
	Nucleotide binding	229 – 232	4	GTP By similarity
	Region	122 – 271	150	G-domain HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	Q2SSE6 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 9DEB5CBD7EE9275C	FASTA	620	68,659
	10 20 30 40 50 60 MKKPVKNIKK QKAQNQTKNI KKQLKEEVNT GLIDGIFVYT EPLSILEFAT KINKPVTVIL 70 80 90 100 110 120 KHYFNQGLLL NQNTLLTEEQ MGELCLEFGF DFKKETSVTK ENILETLLDT VDDEKHLKER 130 140 150 160 170 180 PPIVTIMGHV DHGKTTLLDS IKNSNVVASE AGGITQAIGA YQITTKNNKK ITFIDTPGHE 190 200 210 220 230 240 AFTEMRSRGA NVTDIVVLIV AADDGVMPQT EEAIDHAKLA NVPIIVFINK IDKPGSDPNR 250 260 270 280 290 300 VKTELMKYGL VAEEFGGDIP FIEGSAIKKI NLDKLEDTII LISELENLKA NPDRFASGVV 310 320 330 340 350 360 LEAHLDKAKG PVASVLVQQG SLEIKDIMVV GTTFGSIKHI EDEFKHKVLK AEPSKPVVVY 370 380 390 400 410 420 GLNQVPKAGD KFVVINDEKM AREISEAQLK KQQEEERRTK QAFTLDAIKQ HIDEGELKNI 430 440 450 460 470 480 TLIIKADTQG SVEALKNSLS KINISGVKIN IIRASVGAIS LSDISLASTV RDGLVIVYGF 490 500 510 520 530 540 NVRPDAIVRK KAEEDRIEIR LHNIIYKLIE ELEDAAKGIL DPEIKEVVLG QAQVRALFRH 550 560 570 580 590 600 SAIGTIGGFY VVDGAITRNA KIRVIRNGVV VYDGEINSLQ HQKQDAKEVK AGFEGALTIK 610 620 NFNDIKEGDI FEAYKLEQVK

References

[1]

Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: California kid / ATCC 27343 / NCTC 10154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000123 Genomic DNA. Translation: ABC01401.1.
RefSeq	YP_424317.1. NC_007633.1.
3D structure databases
HSSP	HSSP built from PDB template 1D1N based on UniProtKB P04766.
ProteinModelPortal	Q2SSE6.
ModBase	Search...
Protein-protein interaction databases
STRING	340047.MCAP_0333.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC01401; ABC01401; MCAP_0333.
GeneID	3829005.
KEGG	mcp:MCAP_0333.
PATRIC	20005139. VBIMycCap130493_0338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000076906.
KO	K02519.
OMA	HIGAFTV.
ProtClustDB	PRK05306.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF-2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 1 hit. [Graphical view]
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2_MYCCT

Accession

Primary (citable) accession number: Q2SSE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program