Reviewed, UniProtKB/Swiss-Prot Q2SRN2 (TMFO2_MYCCT)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO 2
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase 2
    Folate-dependent tRNA(M-5-U54)-methyltransferase 2

Gene names

Name:	trmFO2
Ordered Locus Names:	MCAP_0613

Organism

Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]

Taxonomic identifier

340047 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO 2 HAMAP MF_01037		PRO_0000346363
Regions
	Nucleotide binding	8 – 13	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SRN2-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: AE4ADF125AB40FE4	FASTA	423	48,848
	10 20 30 40 50 60 MKTIRIIGAG LSGCEAAYYL LKKGYFVELY EIKTIKKNPI QHYDYFCELA YSDSFRSTDL 70 80 90 100 110 120 NTSVGTLKKE LELLDSLIIK AARYASINQN NELVVNRIEF SKYITNYLKT FNNLKIIEQE 130 140 150 160 170 180 YLNIDLNIPT IIAIGPISTP NFLTNLKKLI NKENLKLFDT VEPTILKQSI NMDICYSLDN 190 200 210 220 230 240 NLNYLYCDLN KEQFEKFYNA LISAKTFNSP LKNEIELLEK NNYFSIESLA KNKQEFINHF 250 260 270 280 290 300 KPINNNAYIT ITLKKDSVIN NLYTIVNFQT NLMWNEQLKV FSLIPGLENL KIMKYGVMHK 310 320 330 340 350 360 NNYINTKKLL NLGVQLKTNK NIFFAGQIIG VDGYVESVCS GLISAINLDR YLNNKKMIIP 370 380 390 400 410 420 NKNSTIGSLY NYLLKTDSNF SPMRINWALV DMIGGFELSD NSKKIYSKRA IKLIKQYLKK INT

References

[1]	Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000123 Genomic DNA. Translation: ABC01649.1.
RefSeq	YP_424581.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3828924.
GenomeReviews	Gene locus MCAP_0613 in contig CP000123_GR.
KEGG	mcp:MCAP_0613.
TIGR	MCAP_0613.
Phylogenomic databases
HOGENOM	Q2SRN2.
OMA	Q2SRN2. IKKNPIQ.
Enzyme and pathway databases
BioCyc	MCAP340047:MCAP_0613-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TMFO2_MYCCT

Accession

Primary (citable) accession number: Q2SRN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)