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Q2SRN2 (TMFO2_MYCCT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO 2
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase 2
Folate-dependent tRNA(M-5-U54)-methyltransferase 2
Gene names
Name:trmFO2
Ordered Locus Names:MCAP_0613
OrganismMycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]
Taxonomic identifier340047 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037

Cofactor

FAD By similarity. HAMAP MF_01037

Subcellular location

Cytoplasm By similarity HAMAP MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO 2 HAMAP MF_01037
PRO_0000346363

Regions

Nucleotide binding8 – 136FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2SRN2 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: AE4ADF125AB40FE4

FASTA42348,848
        10         20         30         40         50         60 
MKTIRIIGAG LSGCEAAYYL LKKGYFVELY EIKTIKKNPI QHYDYFCELA YSDSFRSTDL 

        70         80         90        100        110        120 
NTSVGTLKKE LELLDSLIIK AARYASINQN NELVVNRIEF SKYITNYLKT FNNLKIIEQE 

       130        140        150        160        170        180 
YLNIDLNIPT IIAIGPISTP NFLTNLKKLI NKENLKLFDT VEPTILKQSI NMDICYSLDN 

       190        200        210        220        230        240 
NLNYLYCDLN KEQFEKFYNA LISAKTFNSP LKNEIELLEK NNYFSIESLA KNKQEFINHF 

       250        260        270        280        290        300 
KPINNNAYIT ITLKKDSVIN NLYTIVNFQT NLMWNEQLKV FSLIPGLENL KIMKYGVMHK 

       310        320        330        340        350        360 
NNYINTKKLL NLGVQLKTNK NIFFAGQIIG VDGYVESVCS GLISAINLDR YLNNKKMIIP 

       370        380        390        400        410        420 
NKNSTIGSLY NYLLKTDSNF SPMRINWALV DMIGGFELSD NSKKIYSKRA IKLIKQYLKK 


INT

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References

[1]Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: California kid / ATCC 27343 / NCTC 10154.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000123 Genomic DNA. Translation: ABC01649.1.
RefSeqYP_424581.1. NC_007633.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ2SRN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3828924.
GenomeReviewsGene locus MCAP_0613 in contig CP000123_GR.
KEGGmcp:MCAP_0613.
PATRIC20005703. VBIMycCap130493_0610.
TIGRMCAP_0613.

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHBG285041.
OMACELAYSD.
PhylomeDBQ2SRN2.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycMCAP340047:MCAP_0613-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
[Tree]
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
[Graphical view]
KOK04094.
PfamPF01134. GIDA. 1 hit.
[Graphical view]
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTMFO2_MYCCT
AccessionPrimary (citable) accession number: Q2SRN2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 24, 2006
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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