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Q2SRI9 (SYL_MYCCT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MCAP_0659
OrganismMycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]
Taxonomic identifier340047 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009374

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2SRI9 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: C96DDA009800B974

FASTA80492,983
        10         20         30         40         50         60 
MDFSHKAIEK KWQKYWKENN VYKTTDSNHK KAYVLDMFPY PSGAGLHVGH IKGYTATDVY 

        70         80         90        100        110        120 
SRFKRMQGYD VLHPIGWDAF GLPAEQYALK TGNDPRDFTL KNIENFKAQL VKMGFSYDYD 

       130        140        150        160        170        180 
KEINTADPNY YKVTQWIFKE LYKKGLAENR NIDVNWCQEL GTVLANDEII EKDGLMVSER 

       190        200        210        220        230        240 
GEYPVVKKKM RQWVLKITDY ADKLLKGLDN LDWPNSVKEL QRNWIGKSEG CEINFKSNDI 

       250        260        270        280        290        300 
NIPVFTTRAD TVFGATYIVL APENELVLKL TTPNKLDEVK KYIELTANKS EIERKDESKT 

       310        320        330        340        350        360 
KTGVFIGSYA INPLTKEQIQ IWISDYVLND YGSGAIMAVP AHDKRDWDFA TKFNLPIRFV 

       370        380        390        400        410        420 
ISTKDESKAF VGEGKHINSE FLNDLDRIQS LQVIHDYIEK NNLGKKKTNY KLRDWLFSRQ 

       430        440        450        460        470        480 
RFYGEPFPVL YDKDNNIILV EDDDLPITLP KTDYIKPTNT GESPLANVKN WVNVKIGDRE 

       490        500        510        520        530        540 
YKRETNTMPQ SAGSSWYFIA YILANSKNNL IDLTSDEAKK RLEKWLPVDL YIGGQEHAVG 

       550        560        570        580        590        600 
HLLYSRFWTH FLYDLGLLPT SEPFQRLFNQ GMILGPDNRK MSKSWGNVIN PDDVIDTHGA 

       610        620        630        640        650        660 
DALRLYEMFM GPLDASLPWS FDGLDASLKW LNRCYRMINK IEFSNTNNHK LDYVYNDVVK 

       670        680        690        700        710        720 
KVTQMITELK FNTAISQLMV LVNAIYKEEL STVYKPYIEG FVKMLSLFSP HLAEELWEKL 

       730        740        750        760        770        780 
GNNSSVTLQA WPEFDETKIV KNTVVIALQV NGKLRSTIEV EKGTDKETLI KLAQENENII 

       790        800 
RFIKGHKNLK YIAVVDRIVN IVIE 

« Hide

References

[1]Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: California kid / ATCC 27343 / NCTC 10154.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000123 Genomic DNA. Translation: ABC01150.1.
RefSeqYP_424624.1. NC_007633.1.

3D structure databases

ProteinModelPortalQ2SRI9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340047.MCAP_0659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC01150; ABC01150; MCAP_0659.
GeneID3828466.
KEGGmcp:MCAP_0659.
PATRIC20005795. VBIMycCap130493_0653.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAVNIVTND.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMCAP340047:GC0H-656-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCCT
AccessionPrimary (citable) accession number: Q2SRI9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries