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Q2SRA2 (TRMB_MYCCT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MCAP_0761
OrganismMycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]
Taxonomic identifier340047 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229173

Regions

Region198 – 2014Substrate binding By similarity

Sites

Active site1221 By similarity
Binding site421S-adenosyl-L-methionine By similarity
Binding site671S-adenosyl-L-methionine By similarity
Binding site1221S-adenosyl-L-methionine By similarity
Binding site1261Substrate By similarity
Binding site1581Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2SRA2 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: A6C7373705FAE14B

FASTA22026,151
        10         20         30         40         50         60 
MRLRKKAWTN EFLNQHTYYL IKYDKKINLN EIFLNNNPTC LEIGCGKGQF LTTLALKNPN 

        70         80         90        100        110        120 
LNYIGMEKSS TITAIGLKKS LKEFQNNSKK MTNIKYLNKY AENLLEIFYT DSFSRIYLNF 

       130        140        150        160        170        180 
SDPWPKARHY KKRLTYLGFL DIYSDILIKN GFLEFKTDND SLYNFTLEQL KLTNKWEIIS 

       190        200        210        220 
NTLDLYNDQE LLKDNVPTEY ETKFHLANKN IYKIVIKNLK 

« Hide

References

[1]Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: California kid / ATCC 27343 / NCTC 10154.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000123 Genomic DNA. Translation: ABC01304.1.
RefSeqYP_424711.1. NC_007633.1.

3D structure databases

ProteinModelPortalQ2SRA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340047.MCAP_0761.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC01304; ABC01304; MCAP_0761.
GeneID3828895.
KEGGmcp:MCAP_0761.
PATRIC20005999. VBIMycCap130493_0741.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAINYLEAT.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycMCAP340047:GC0H-758-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCCT
AccessionPrimary (citable) accession number: Q2SRA2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 24, 2006
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways