Q2SPQ1 (CHEB1_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	HCH_00463

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264281
Regions
	Domain	10 – 127	118	Response regulatory
	Domain	167 – 359	193	CheB-type methylesterase
Sites
	Active site	179	1	By similarity
	Active site	205	1	By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Modified residue	61	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SPQ1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 254F5C128A8D3941	FASTA	361	39,023
	10 20 30 40 50 60 MSSGAGKVYK VLVVDDSALM RKILVEILNQ SPCLEVVDTA GDPYQARDKI KALSPDVITL 70 80 90 100 110 120 DVEMPRMDGL TFLRNLMRLR PMPVVMISSL TEQGAAVTLD ALEYGAVDFI AKPKIDLREG 130 140 150 160 170 180 IEEKAQEIID KVITAARIPH DKLLLKQQRL QTQHQKAAVG ERPQPVFATT DKLIAVGAST 190 200 210 220 230 240 GGLDAIRDLL DGLNMDLPGI VIAQHIPGAF SRSFAERLDR RLPMKVEEAY DNAPITMGRV 250 260 270 280 290 300 YIAPGEYHLE VVRTGAKYVC RLSEAPPVNR HRPSVDVLFD SIVSAAGANA MAALLTGMGK 310 320 330 340 350 360 DGAAGLLRLH EAGAYTIAQD EASSVVWGMP GAAVKLNAAD DVLPLEKIAA SVKRWYAENP G

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed: 16352867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KCTC 2396.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC27373.1.
RefSeq	YP_431798.1. NC_007645.1.
3D structure databases
ProteinModelPortal	Q2SPQ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SPQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3840343.
GenomeReviews	Gene locus HCH_00463 in contig CP000155_GR.
KEGG	hch:HCH_00463.
NMPDR	fig|349521.5.peg.435.
PATRIC	22082062. VBIHahChe29232_0439.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PPSVADF.
PhylomeDB	Q2SPQ1.
Enzyme and pathway databases
BioCyc	HCHE349521:HCH_00463-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_HAHCH

Accession

Primary (citable) accession number: Q2SPQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program