Reviewed, UniProtKB/Swiss-Prot Q2SPF9 (PCKA_HAHCH)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxykinase [ATP]
      Short name=PEP carboxykinase
      Short name=PEPCK
    EC=4.1.1.49
Alternative name(s):
    Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	HCH_00561

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Phosphoenolpyruvate carboxykinase [ATP] HAMAP MF_00453		PRO_0000236926
Regions
	Nucleotide binding	216 – 223	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SPF9-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: B38E210BBD92A090	FASTA	511	56,682
	10 20 30 40 50 60 MSNIYTDLSS AELVEQAISR KEGRMAANGS LVVNTGKRTG RSPMDRFIVQ EPSTADAIDW 70 80 90 100 110 120 GAINRPFDAE KFDALWDRVE DYISTRDRFV SHLHVGADPE HYLPIKVTTE TAWHNLFGRN 130 140 150 160 170 180 LFIRPERYNP ADKQEWQVMN IPDFVCEPER DGTNSDGAVI INFARRKVLL AGMRYAGEMK 190 200 210 220 230 240 KAMFSVQNFL LPEKDVLPMH CSANVGEDGQ TCLFFGLSGT GKTTLSADES RFLIGDDEHG 250 260 270 280 290 300 WGRGTVFNVE GGCYAKCIDL SQKNEPVIWN AIRFGAIVEN VVIDDQDRTP DYTDVSLTEN 310 320 330 340 350 360 TRCAYPLEHV EKRVLENRAG EPSAIVFLTC DMTGVLPPVS ILSKEAAAYH FLSGYTALVG 370 380 390 400 410 420 STEMGSSSKL KSTFSTCFGA PFFPRPAGVY AELLMKRMEE FGSKVYLVNT GWTGGPHGVG 430 440 450 460 470 480 KRFSIPVTRA IISAIQSGEL ADVETEHLEK LNLTVPKELE GVDSVLLNPR NTWADKEAYD 490 500 510 AKAQDLIQQF VENFKKFDVS DAIRAAGPSL D

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed: 16352867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000155 Genomic DNA. Translation: ABC27465.1.
RefSeq	YP_431890.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3839670.
GenomeReviews	Gene locus HCH_00561 in contig CP000155_GR.
KEGG	hch:HCH_00561.
NMPDR	fig|349521.5.peg.520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2SPF9.
OMA	Q2SPF9. ELAWHSL.
Enzyme and pathway databases
BioCyc	HCHE349521:HCH_00561-MON.
Family and domain databases
HAMAP	MF_00453. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
ProDom	PD004738. PEPCK_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00224. pckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_HAHCH

Accession

Primary (citable) accession number: Q2SPF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)