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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Hahella chejuensis (strain KCTC 2396)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:HCH_01044
OrganismiHahella chejuensis (strain KCTC 2396)
Taxonomic identifieri349521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella
Proteomesi
  • UP000000238 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001339061 – 963Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST963

Interactioni

Protein-protein interaction databases

STRINGi349521.HCH_01044.

Structurei

3D structure databases

ProteinModelPortaliQ2SN48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 451Adenylyl removaseUniRule annotationAdd BLAST451
Regioni461 – 963Adenylyl transferaseUniRule annotationAdd BLAST503

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG090J01K0.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q2SN48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSELQLY PSVIHPAIQK YWDEFCELPA ETLSAFSSYY PDILRAFAVS
60 70 80 90 100
DFLALNILRE PELVLSFLQS SQHQKECQLV DFRAPLAEEL SGALKEDDLL
110 120 130 140 150
RILRLFRRRM MFRIIWRDLV CLVDYQVTTR EVSWLAETCI DEALSWIYRD
160 170 180 190 200
MSQQFGEPHS TAGVKQQLVV LAMGKLGANE LNVSSDIDLI FTFPERGETQ
210 220 230 240 250
GGNRSLDNQA FFTRLGQRLI QALDNTTADG FVFRVDMRLR PYGQSGALAL
260 270 280 290 300
SFAAMEAYYQ EQGRDWERYA MIKARAVAGD IDAGEELLTS LKPFVYRKYI
310 320 330 340 350
DFGAVQALRA MKEMIEREVQ RKGQDGNIKV GRGGIREIEF IVQVFQLMRG
360 370 380 390 400
GRDVRLQQRN LLSTLECLED EGLLPVAAAA ELREAYIFLR NLEHAIQGLE
410 420 430 440 450
DKQTQEIPKD EEGRERVAVA MGFPGWGACR VQLDKYRDCV SEHFADIIAE
460 470 480 490 500
RREAPLERPD TIEWKALWAG RLAQDEAQDL LLRSGCDQAQ EIYEAVTQLR
510 520 530 540 550
QSSRVQHMQL QGKERLDEFM PNLLQSLAQS RASHETCRRL LALVEAVLRR
560 570 580 590 600
SAYLALLNEN PGALTELIRL FAESAWIARQ IVTTPLLLDE LLHSGSLYTP
610 620 630 640 650
PDTTALRDEL RQQMLRIPND DLEEQMEALR YFKKAHVLRV AASDLRETLP
660 670 680 690 700
LMKVSDYLTF IAETIIEQVV DEAWETMVKK YGEPGYEDAA LNDMRFAVIG
710 720 730 740 750
YGKLGGIEMS YGSDLDLVFL HGASGDLSTS GETSIANQVF FTRLGQRIIH
760 770 780 790 800
LLTTKTASGD IYEVDMRLRP SGNSGLLVSS VSAFQQYQEK SAWTWEHQAL
810 820 830 840 850
VRSRAVAGCP GIRAEFEKVR KDILTQHRDP AKLRTEVMDM REKMHASLGT
860 870 880 890 900
KPDSSGSPPY FDLKHDRGGI VDIEFMVQYA ALRWANEYPQ IVVWSDNIRI
910 920 930 940 950
LEALGDAGVF SQEDADKLCD IYRLLRAHGH RLALQNLPAR IQTDELLEER
960
RWVVAFWEKV FAE
Length:963
Mass (Da):109,664
Last modified:January 24, 2006 - v1
Checksum:i3A6E3E86D9B9328C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA. Translation: ABC27926.1.
RefSeqiWP_011395001.1. NC_007645.1.

Genome annotation databases

EnsemblBacteriaiABC27926; ABC27926; HCH_01044.
KEGGihch:HCH_01044.
PATRICi22083124. VBIHahChe29232_0970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA. Translation: ABC27926.1.
RefSeqiWP_011395001.1. NC_007645.1.

3D structure databases

ProteinModelPortaliQ2SN48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349521.HCH_01044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC27926; ABC27926; HCH_01044.
KEGGihch:HCH_01044.
PATRICi22083124. VBIHahChe29232_0970.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG090J01K0.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_HAHCH
AccessioniPrimary (citable) accession number: Q2SN48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 24, 2006
Last modified: February 15, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.