Reviewed, UniProtKB/Swiss-Prot Q2SLT4 (SAHH_HAHCH)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	HCH_01532

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024728
Regions
	Region	191 – 373	183	NAD binding By similarity
Sites
	Binding site	63	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	198	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SLT4-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 40D703C8EAE28FF6	FASTA	463	51,348
	10 20 30 40 50 60 MAQLQSVDNF SDYKVKDISL AAWGRKEIDI AEGEMPALMT LREKYRAQQP LAGARILGCI 70 80 90 100 110 120 HMTIQTAVLI ETLVALGAEV RWSSCNIFST QDHAAAAIAA AGVPVFAWKG ETEEEYVWCI 130 140 150 160 170 180 KQTITKDGQP WNANMVLDDG GDLTEILHNE FPQMLDHIHG ISEETTTGVH RLLDMMKKGE 190 200 210 220 230 240 LKVPAVNVND SVTKSKNDNK YGCRHSLNDA IKRATDHLLA GKKALVIGYG DVGKGSAASL 250 260 270 280 290 300 RQEGMIVKIS EIDPICAMQA CMDGYEVVSP YIDGVNTGAA DGVNRDLLGH TDLLVTTTGN 310 320 330 340 350 360 VNVCDKYMLQ ALKSGAVVCN IGHFDNEIDT RFMRDNWEWE EVKPQVHVIY RNKDQNDHLL 370 380 390 400 410 420 LLSEGRLVNL GNATGHPSRI MDGSFANQVL AQMYLFERKF ADLPADEKPK NLYVRVLPKQ 430 440 450 460 LDEEVARYMV QGFGGVITKL TQPQAKYIGV EVEGPYKPTD YKY

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed: 16352867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC28390.1.
RefSeq	YP_432815.1.
3D structure databases
HSSP	HSSP built from PDB template 1K0U based on UniProtKB P10760.
SMR	Q2SLT4. Positions 10-463.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SLT4.
Genome annotation databases
GeneID	3837562.
GenomeReviews	Gene locus HCH_01532 in contig CP000155_GR.
KEGG	hch:HCH_01532.
NMPDR	fig|349521.5.peg.1378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0499.
HOGENOM	HBG352029.
OMA	VITHDHM.
PhylomeDB	Q2SLT4.
Enzyme and pathway databases
BioCyc	HCHE349521:HCH_01532-MONOMER.
Family and domain databases
HAMAP	MF_00563_B. AdoHcyase_B. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_HAHCH

Accession

Primary (citable) accession number: Q2SLT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)