Reviewed, UniProtKB/Swiss-Prot Q2SJZ6 (DNAE2_HAHCH)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	HCH_02200

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella

Protein attributes

Sequence length	1032 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1032	1032	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000073690

Sequences

	Sequence		Length	Mass (Da)
	Q2SJZ6-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 9D5BC44F91C8D5C2	FASTA	1,032	117,543
	10 20 30 40 50 60 MSFVELHCIS NYSFLKGASH PEELVRQAIA QGYEGLAITD ECSVAGAVKA WRELRRLRQE 70 80 90 100 110 120 APEASAFKLI IGSEFHYEGN CFVVLAPHKK AYGELCRFIT DCRRKADKGD YQFSPDALNE 130 140 150 160 170 180 NIKQGLLLWR PKQSDQEFIP ALTKIFSGRL WLLLELSLSE EDDSERELIQ TLSSAYNLPV 190 200 210 220 230 240 VSSNGVKMHA PERKRLHDAL TAIRFNQSVD SIKDRLAPNA ENYLRPLNEI HEIYPSFTIQ 250 260 270 280 290 300 ESLRIAAKCA FELNEIRYQY PKEVVPEGDD PSAYLRRLTY EGAHLRYPQG IPENVLATLE 310 320 330 340 350 360 KELNIISELQ YEYYFLTIFD IVDYAKKSQI LCQGRGSAAN SVVCYCLGIT AVDPTRASLL 370 380 390 400 410 420 FERFISKGRD EPPDIDVDFE NARREEVIQY LYRRYGRERC AIAATVITYR PKSAIRDLGK 430 440 450 460 470 480 ALGVDLLQLE SVIANYGWRY RGQDWIDEVI TPQVSQDNHI LTCFKELLPE LLGFPRHLSQ 490 500 510 520 530 540 HVGGFVLSAG PLVELVPIEN AAMEDRTVIQ WDKDDLESLG LMKVDVLALG MLTALKKCTT 550 560 570 580 590 600 YISEITGKTF SLESIPKEED SQVYDMLQRA DTVGLFQVES RAQMNMLPRL RPEKYYDLVV 610 620 630 640 650 660 QVAIVRPGPI HGDMVHPYLR RRHNLEDPDD VPLPELKPIL KRTFGVPIFQ EQVIAIAIVG 670 680 690 700 710 720 AGFTSEEAEE LRRSMASWKR RGHMGKLMDK LIINLLKKGV PLEYIQRLCR QIEGFGEYGF 730 740 750 760 770 780 PESHAASFAL LAYHSGWLKY YYPSAFCCAL LNSQPMGFYA PWQVIQDAQR HGVIVLPVDI 790 800 810 820 830 840 NNSYWEHRLE PHSDNTKEGA LRLGFRLVKG LSEEAACSII QHRTDEGFTS LVQVMHLPKI 850 860 870 880 890 900 NREDLEALAS ANALASLGEN RYQQRWECSG FLYYYQLFSE CEYINIDFEP PSRLDNIYED 910 920 930 940 950 960 HSSTGVVLND HPLAYLREAN LTPQCLTAVE LLKQKAGLKT YVAGVVINRQ RPKTSTGVTF 970 980 990 1000 1010 1020 VTLEDETGSI NILVWKKTAI AQMDILVKAR LLMVYGELDK DEEGRVAHVL AHRLTDLTPH 1030 LEELESRSRD FH

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed: 16352867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC29028.1.
RefSeq	YP_433453.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SJZ6.
Genome annotation databases
GeneID	3837765.
GenomeReviews	Gene locus HCH_02200 in contig CP000155_GR.
KEGG	hch:HCH_02200.
NMPDR	fig|349521.5.peg.1977.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	LATNRNG.
Enzyme and pathway databases
BioCyc	HCHE349521:HCH_02200-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_HAHCH

Accession

Primary (citable) accession number: Q2SJZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 24, 2006
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)