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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Hahella chejuensis (strain KCTC 2396)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei84Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei151Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei484Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei533Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei560Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei796Substrate; via amide nitrogenUniRule annotation1
Binding sitei804Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei821Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHCHE349521:G1G5E-3038-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HCH_03364
OrganismiHahella chejuensis (strain KCTC 2396)
Taxonomic identifieri349521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella
Proteomesi
  • UP000000238 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_000025607331 – 830Periplasmic nitrate reductaseUniRule annotationAdd BLAST800

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi349521.HCH_03364

Structurei

3D structure databases

ProteinModelPortaliQ2SGV7
SMRiQ2SGV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 964Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 220Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni244 – 248Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni510 – 511Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni720 – 729Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2SGV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRREFIKR SAAVTAACTA GISLSGEASN VITDSEYTRL KWSKAPCRFC
60 70 80 90 100
GTGCSVNVAV KDNQVVATHG DIQSEVNRGL NCVKGYFLSK IMYGKDRLTQ
110 120 130 140 150
PLLRKKNGEY HKDGDFTPVT WDEAFDVMAK QFKKTLKEKG PTAVGMFGSG
160 170 180 190 200
QWTVWEGYAA VKLYKAGFRS NNIDPNARHC MASAVAGFMR TFGIDEPMGC
210 220 230 240 250
YDDIEHADAF VLWGSNMAEM HPILWTRVTD RRLSHPHVKV AVLSTFQHRC
260 270 280 290 300
FDLADLPIIF TPQADLAILN YIARYIIEKD MVNWDFVNKH VRFKVGAADI
310 320 330 340 350
GYGLRPEHQL ELAAANASNP GGAKDSSFEE YKNFLQQYDA QFVSKLSGAS
360 370 380 390 400
KEKLDQLAEL YANPKTRVTS FWTMGFNQHT RGVWCNNLVY NLHLLTGKIS
410 420 430 440 450
SPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADMVVTNPKH PEFAEKTWKI
460 470 480 490 500
PPGVIPDKPG YHAVLQNRKL RDGELNAYWV QVNNNVQAAP NMLEETLPGY
510 520 530 540 550
RNPNNFIVVS EAYPTVTSQA ADLILPAAMW VEKEGAYGNA ERRTQFWRQL
560 570 580 590 600
VSAPGEAKSD LWQIMEFSKR FTTDEVWPEE ILSKSPEFKG KSLFDVLFAN
610 620 630 640 650
NSVNKYSIEE IDPNYKNHES EHFGFYVQKG LFEEYAIFGR GHGHDLAEFD
660 670 680 690 700
RYHEARGLRW PVVDGKETLW RFREGSDPYV KKGAGYQFYG HSDGKAIIFA
710 720 730 740 750
LPYEPPAESP DQDYPYWLVT GRVLEHWHSG SMTQRVPELY LAVPDALVYM
760 770 780 790 800
HPDDARKLGV RRGDEIKVVS RRGEMRSRVE TRGRNKPPVG LVFVPWFDAS
810 820 830
QLINKCTLDA TDPISKQTDF KKCAVKLIKV
Length:830
Mass (Da):93,708
Last modified:January 24, 2006 - v1
Checksum:iC120B7A0EC56DAB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA Translation: ABC30117.1
RefSeqiWP_011397186.1, NC_007645.1

Genome annotation databases

EnsemblBacteriaiABC30117; ABC30117; HCH_03364
KEGGihch:HCH_03364

Similar proteinsi

Entry informationi

Entry nameiNAPA_HAHCH
AccessioniPrimary (citable) accession number: Q2SGV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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