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Q2SGV7 (NAPA_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:HCH_03364
OrganismHahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]
Taxonomic identifier349521 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 830800Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000256073

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2SGV7 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: C120B7A0EC56DAB8

FASTA83093,708
        10         20         30         40         50         60 
MTTRREFIKR SAAVTAACTA GISLSGEASN VITDSEYTRL KWSKAPCRFC GTGCSVNVAV 

        70         80         90        100        110        120 
KDNQVVATHG DIQSEVNRGL NCVKGYFLSK IMYGKDRLTQ PLLRKKNGEY HKDGDFTPVT 

       130        140        150        160        170        180 
WDEAFDVMAK QFKKTLKEKG PTAVGMFGSG QWTVWEGYAA VKLYKAGFRS NNIDPNARHC 

       190        200        210        220        230        240 
MASAVAGFMR TFGIDEPMGC YDDIEHADAF VLWGSNMAEM HPILWTRVTD RRLSHPHVKV 

       250        260        270        280        290        300 
AVLSTFQHRC FDLADLPIIF TPQADLAILN YIARYIIEKD MVNWDFVNKH VRFKVGAADI 

       310        320        330        340        350        360 
GYGLRPEHQL ELAAANASNP GGAKDSSFEE YKNFLQQYDA QFVSKLSGAS KEKLDQLAEL 

       370        380        390        400        410        420 
YANPKTRVTS FWTMGFNQHT RGVWCNNLVY NLHLLTGKIS SPGNSPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFAHRLP ADMVVTNPKH PEFAEKTWKI PPGVIPDKPG YHAVLQNRKL RDGELNAYWV 

       490        500        510        520        530        540 
QVNNNVQAAP NMLEETLPGY RNPNNFIVVS EAYPTVTSQA ADLILPAAMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQFWRQL VSAPGEAKSD LWQIMEFSKR FTTDEVWPEE ILSKSPEFKG KSLFDVLFAN 

       610        620        630        640        650        660 
NSVNKYSIEE IDPNYKNHES EHFGFYVQKG LFEEYAIFGR GHGHDLAEFD RYHEARGLRW 

       670        680        690        700        710        720 
PVVDGKETLW RFREGSDPYV KKGAGYQFYG HSDGKAIIFA LPYEPPAESP DQDYPYWLVT 

       730        740        750        760        770        780 
GRVLEHWHSG SMTQRVPELY LAVPDALVYM HPDDARKLGV RRGDEIKVVS RRGEMRSRVE 

       790        800        810        820        830 
TRGRNKPPVG LVFVPWFDAS QLINKCTLDA TDPISKQTDF KKCAVKLIKV 

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References

[1]"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent."
Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F.
Nucleic Acids Res. 33:7066-7073(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KCTC 2396.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000155 Genomic DNA. Translation: ABC30117.1.
RefSeqYP_434542.1. NC_007645.1.

3D structure databases

ProteinModelPortalQ2SGV7.
SMRQ2SGV7. Positions 40-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349521.HCH_03364.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC30117; ABC30117; HCH_03364.
GeneID3838263.
KEGGhch:HCH_03364.
PATRIC22087390. VBIHahChe29232_3068.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycHCHE349521:GHAL-3229-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_HAHCH
AccessionPrimary (citable) accession number: Q2SGV7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families