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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Hahella chejuensis (strain KCTC 2396)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHCHE349521:GHAL-3229-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HCH_03364
OrganismiHahella chejuensis (strain KCTC 2396)
Taxonomic identifieri349521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella
ProteomesiUP000000238: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signalUniRule annotationAdd
BLAST
Chaini31 – 830800Periplasmic nitrate reductasePRO_0000256073Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi349521.HCH_03364.

Structurei

3D structure databases

ProteinModelPortaliQ2SGV7.
SMRiQ2SGV7. Positions 40-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiRIFALPY.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2SGV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTRREFIKR SAAVTAACTA GISLSGEASN VITDSEYTRL KWSKAPCRFC
60 70 80 90 100
GTGCSVNVAV KDNQVVATHG DIQSEVNRGL NCVKGYFLSK IMYGKDRLTQ
110 120 130 140 150
PLLRKKNGEY HKDGDFTPVT WDEAFDVMAK QFKKTLKEKG PTAVGMFGSG
160 170 180 190 200
QWTVWEGYAA VKLYKAGFRS NNIDPNARHC MASAVAGFMR TFGIDEPMGC
210 220 230 240 250
YDDIEHADAF VLWGSNMAEM HPILWTRVTD RRLSHPHVKV AVLSTFQHRC
260 270 280 290 300
FDLADLPIIF TPQADLAILN YIARYIIEKD MVNWDFVNKH VRFKVGAADI
310 320 330 340 350
GYGLRPEHQL ELAAANASNP GGAKDSSFEE YKNFLQQYDA QFVSKLSGAS
360 370 380 390 400
KEKLDQLAEL YANPKTRVTS FWTMGFNQHT RGVWCNNLVY NLHLLTGKIS
410 420 430 440 450
SPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADMVVTNPKH PEFAEKTWKI
460 470 480 490 500
PPGVIPDKPG YHAVLQNRKL RDGELNAYWV QVNNNVQAAP NMLEETLPGY
510 520 530 540 550
RNPNNFIVVS EAYPTVTSQA ADLILPAAMW VEKEGAYGNA ERRTQFWRQL
560 570 580 590 600
VSAPGEAKSD LWQIMEFSKR FTTDEVWPEE ILSKSPEFKG KSLFDVLFAN
610 620 630 640 650
NSVNKYSIEE IDPNYKNHES EHFGFYVQKG LFEEYAIFGR GHGHDLAEFD
660 670 680 690 700
RYHEARGLRW PVVDGKETLW RFREGSDPYV KKGAGYQFYG HSDGKAIIFA
710 720 730 740 750
LPYEPPAESP DQDYPYWLVT GRVLEHWHSG SMTQRVPELY LAVPDALVYM
760 770 780 790 800
HPDDARKLGV RRGDEIKVVS RRGEMRSRVE TRGRNKPPVG LVFVPWFDAS
810 820 830
QLINKCTLDA TDPISKQTDF KKCAVKLIKV
Length:830
Mass (Da):93,708
Last modified:January 24, 2006 - v1
Checksum:iC120B7A0EC56DAB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA. Translation: ABC30117.1.
RefSeqiYP_434542.1. NC_007645.1.

Genome annotation databases

EnsemblBacteriaiABC30117; ABC30117; HCH_03364.
GeneIDi3838263.
KEGGihch:HCH_03364.
PATRICi22087390. VBIHahChe29232_3068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA. Translation: ABC30117.1.
RefSeqiYP_434542.1. NC_007645.1.

3D structure databases

ProteinModelPortaliQ2SGV7.
SMRiQ2SGV7. Positions 40-827.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349521.HCH_03364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC30117; ABC30117; HCH_03364.
GeneIDi3838263.
KEGGihch:HCH_03364.
PATRICi22087390. VBIHahChe29232_3068.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiRIFALPY.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciHCHE349521:GHAL-3229-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KCTC 2396.

Entry informationi

Entry nameiNAPA_HAHCH
AccessioniPrimary (citable) accession number: Q2SGV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.