Q2SFY4 (CHEB2_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 2
EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	HCH_03703

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000264282
Regions
	Domain	6 – 123	118	Response regulatory
	Domain	169 – 361	193	CheB-type methylesterase
Sites
	Active site	181	1	By similarity
	Active site	207	1	By similarity
	Active site	303	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SFY4 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 3391E8B49206B93B	FASTA	368	38,865
	10 20 30 40 50 60 MNAKIKVLLV DDSAVVRQVL QQVLERDKNI EVIGAAADPI FAQLRMSKQW PDVIVLDVEM 70 80 90 100 110 120 PRMDGITFLK KLMAERPTPV VMCSTLTSKG AQTSMKALAA GAVAVVAKPA VGVKEYLESA 130 140 150 160 170 180 AGELIMEIKA AAKANMRNMP AATASAQSMA LPAKFSADAV IPLRTGGVIA GANKIAALGT 190 200 210 220 230 240 STGGTQALEY VLTRLPRNCP GIVIVQHMPE KFTAAFAERL NSICELEILE AESGHQVAPG 250 260 270 280 290 300 RALIAPGGKH MLLRRSGAQY FVEIMSGPPV NRHRPSVDVL FRSVAQAAGR NALGVIMTGM 310 320 330 340 350 360 GDDGAKGLLE MRNAGALTIG QDERSCIVYG MPKEAAKIGA VTREISLERI PQCVLDMGAS LTASAQRG

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed: 16352867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KCTC 2396.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC30440.1.
RefSeq	YP_434865.1. NC_007645.1.
3D structure databases
ProteinModelPortal	Q2SFY4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SFY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3842810.
GenomeReviews	Gene locus HCH_03703 in contig CP000155_GR.
KEGG	hch:HCH_03703.
NMPDR	fig|349521.5.peg.3259.
PATRIC	22087990. VBIHahChe29232_3368.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	Q2SFY4.
Enzyme and pathway databases
BioCyc	HCHE349521:HCH_03703-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_HAHCH

Accession

Primary (citable) accession number: Q2SFY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program