Phenylalanine--tRNA ligase beta subunit - Hahella chejuensis (strain KCTC 2396)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	HCH_04574

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella ›

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00283
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 790

790

Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283

PRO_0000232801

Regions

Domain

39 – 147

109

tRNA-binding

Domain

400 – 476

77

B5

Domain

696 – 789

94

FDX-ACB

Sites

Metal binding

454

1

Magnesium By similarity

Metal binding

460

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

463

1

Magnesium By similarity

Metal binding

464

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q2SDJ7 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: E9BB2827187612F9
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FASTA

790

86,900

        10         20         30         40         50         60 
MKFSEYWLRE WVDPQLSSEA LVQQITMAGL EVDAAERVAK PFSGIVVGEV LSVEPHPDAD 

        70         80         90        100        110        120 
KLRVCKVSDG QEEFQVVCGA PNVAAGMKVP FAKIGAVLSG DFKIKKAKLR GVESQGMLCS 

       130        140        150        160        170        180 
EEELTLAEKS DGLMPLAADA PVGVDVRSYL KLDDNIIDVD LTPNRSDCLS ILGMAREVAA 

       190        200        210        220        230        240 
LNKIPFEAPE VKKTAAVIDD VFPVRVEAPE ACPRYVGRVI KGVDLSAPTP QWMVEKLRRS 

       250        260        270        280        290        300 
GIRSIDAVVD VTNFVMIELG QPMHAFDLNE LADGIVVRMP TPGEKLVLLD GQEITINPDT 

       310        320        330        340        350        360 
LVIADNEKPL ALAGVMGGEH SGVSEKTKDI FLESAFFAPL HLAGKARSYG LHTDSSHRFE 

       370        380        390        400        410        420 
RGVDYAHQAR AIERATELLL EIVGGQPGPV TEICSKEHLP AKALVNLRHA RVDELLGVHL 

       430        440        450        460        470        480 
DRTRVEEMLA RLGLGVHKVA KDGWTFSAPT HRFDISIEVD LIEEVARLYG YNKLPVTEPM 

       490        500        510        520        530        540 
APLGIKPLPE SKVSLRRLKL HLVSRGYQEA ITYTFVDQKV LSLLTPEEDT ITLANPIASD 

       550        560        570        580        590        600 
MAAMRTTLWA GLLQTALYNQ NRQQNRIRLF ESGLRFLKRG GQIQQDPMLA GLIMGASMPE 

       610        620        630        640        650        660 
GWESKKRTVD FYDVKGDLES LLEIPGCNLS FRSAQHPALH PGQTAEVLKD GQPIGVLGAL 

       670        680        690        700        710        720 
HPRIAKTLNL NGPLYLFELC LNSIADGQVP SFKGVSRFPE IRRDLAIVID EQTPYADVAL 

       730        740        750        760        770        780 
AIAQSAGEWR VHHELFDVYH GESVGADKKS LAISIVWRHP ERTLRDEEIT EAFDNVVKEL 

       790 
GNRFGASLRS

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References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent."
Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F.
Nucleic Acids Res. 33:7066-7073(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KCTC 2396.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC31277.1.
RefSeq	YP_435702.1. NC_007645.1.
3D structure databases
ProteinModelPortal	Q2SDJ7.
ModBase	Search...
Protein-protein interaction databases
STRING	349521.HCH_04574.
Proteomic databases
PRIDE	Q2SDJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3843562.
KEGG	hch:HCH_04574.
PATRIC	22089564. VBIHahChe29232_4151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0073.
HOGENOM	HOG000292087.
KO	K01890.
OMA	MKFSEQW.
ProtClustDB	PRK00629.
Enzyme and pathway databases
BioCyc	HCHE349521:GHAL-4393-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.56.20. 1 hit. 3.30.70.380. 1 hit. 3.50.40.10. 1 hit.
HAMAP	MF_00283. Phe_tRNA_synth_beta1.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. pheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_HAHCH

Accession

Primary (citable) accession number: Q2SDJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	January 24, 2006
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents