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Q2SBE7 (SYL_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:HCH_05355
OrganismHahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]
Taxonomic identifier349521 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009351

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif620 – 6245"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2SBE7 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: F240E4CD127901D7

FASTA86198,380
        10         20         30         40         50         60 
MEELYHPKKV EQEAQKFWEE TDAFKVDEEP GKEKFYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMQG KNVLQPMGWD AFGLPAENAA LKNNVAPAKW TYENIAYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WNRELATCRP EYYKWEQWFF TRLYEKGLVY KKMSTVNWDP VDETVLANEQ VIDGKGWRSG 

       190        200        210        220        230        240 
AVVERREIPQ WFIKITDYAE QLLNDLDKLE NWPEQVKTMQ RNWINKSEGV EFHFKLKDHD 

       250        260        270        280        290        300 
GDLQVYTTRP DTIMGVTYVA IAPQHPLALE AAASNPQLAK FLDECKNTKV AEADMATMEK 

       310        320        330        340        350        360 
RGMDTGFFVI HPLTNEPAPI WVANFVLMDY GSGAVMSVPG HDERDHEFAL KYGLPIKQVI 

       370        380        390        400        410        420 
SVIDADEVDI QEKAITEKGI LVNSGEFTGM TSQEAFDAVA EKLTAMGIGE KKINYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMMTQEDG TEVPVPLADL PVRLPEDVEM DGVKSPIKAD PNWSKRSYNG 

       490        500        510        520        530        540 
QPATQETDTF DTFMESSWYY ARFCCPNLES AMLDPAAANY WLPVDYYVGG IEHAILHLLY 

       550        560        570        580        590        600 
SRFFHKLLRD EGLVDSDEPF KKLLCQGMVI AETFYREDPS SKKTYFNPRD VRVELDTQGN 

       610        620        630        640        650        660 
SPKAFLIEDG QPVIIGPKEK MSKSKNNGVD PQELIDKYGA DTVRLFTMFA APPEQSLEWS 

       670        680        690        700        710        720 
ESGVEGQHRF LRRLWKLVHT HVEKKGAAPL NVAELNETQR TLRRKTHETI KKMTDEFNHR 

       730        740        750        760        770        780 
MAINTGIAMV MELLNELSRF EDDSPQGLAV VQEALETAVL VLAPIVPHIS HGLWRELGHE 

       790        800        810        820        830        840 
DVVMNAPWPQ LDEKALEKDS IELVVQVNGK LRARIQAAAS ANKDELEKLA FDDENVQRFM 

       850        860 
EDKTVVKVIV VPGKLVNIVV K 

« Hide

References

[1]"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent."
Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F.
Nucleic Acids Res. 33:7066-7073(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KCTC 2396.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000155 Genomic DNA. Translation: ABC32027.1.
RefSeqYP_436452.1. NC_007645.1.

3D structure databases

ProteinModelPortalQ2SBE7.
SMRQ2SBE7. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349521.HCH_05355.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC32027; ABC32027; HCH_05355.
GeneID3840473.
KEGGhch:HCH_05355.
PATRIC22091050. VBIHahChe29232_4876.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHCHE349521:GHAL-5161-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HAHCH
AccessionPrimary (citable) accession number: Q2SBE7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries