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Q2SBD4

- BIOB_HAHCH

UniProt

Q2SBD4 - BIOB_HAHCH

Protein

Biotin synthase

Gene

bioB

Organism
Hahella chejuensis (strain KCTC 2396)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi99 – 991Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi190 – 1901Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi262 – 2621Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciHCHE349521:GHAL-5174-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:HCH_05371
    OrganismiHahella chejuensis (strain KCTC 2396)
    Taxonomic identifieri349521 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella
    ProteomesiUP000000238: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Biotin synthasePRO_0000381420Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi349521.HCH_05371.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2SBD4.
    SMRiQ2SBD4. Positions 6-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2SBD4-1 [UniParc]FASTAAdd to Basket

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    MQSPIRHDWS LEEVEALFAL PFNDLLFQAQ VTHRRNFDPN EVQVSTLLSI    50
    KTGACPEDCK YCPQSGHYNT GLEKEKLLEV EKVVNEAKAA KEKGASRFCM 100
    GAAWRNPRAK DMPYVLEMVK QVKSLGMETC MTLGMLTADQ ASELAGAGLD 150
    YYNHNLDTSE NYYGEIITTR TYSDRLETLQ NVRDAGMKVC CGGIMGMGES 200
    ARDRAALLAQ LANLPSHPES VPINMLVKVK GTPLEVQEDI DPFDFIRTIA 250
    VARILMPRSH VRLSAGREDM NEQMQAMCFL AGANSIFYGE KLLTTPNPEA 300
    DKDMMLFKKL GIRPEQREQA HSEDQQEAYL HDVIQAHDQQ QRMESMFYDA 350
    TKTA 354
    Length:354
    Mass (Da):39,840
    Last modified:January 24, 2006 - v1
    Checksum:i5A87E0B12D896F00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000155 Genomic DNA. Translation: ABC32040.1.
    RefSeqiWP_011399104.1. NC_007645.1.
    YP_436465.1. NC_007645.1.

    Genome annotation databases

    EnsemblBacteriaiABC32040; ABC32040; HCH_05371.
    GeneIDi3840602.
    KEGGihch:HCH_05371.
    PATRICi22091080. VBIHahChe29232_4891.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000155 Genomic DNA. Translation: ABC32040.1 .
    RefSeqi WP_011399104.1. NC_007645.1.
    YP_436465.1. NC_007645.1.

    3D structure databases

    ProteinModelPortali Q2SBD4.
    SMRi Q2SBD4. Positions 6-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349521.HCH_05371.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC32040 ; ABC32040 ; HCH_05371 .
    GeneIDi 3840602.
    KEGGi hch:HCH_05371.
    PATRICi 22091080. VBIHahChe29232_4891.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci HCHE349521:GHAL-5174-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KCTC 2396.

    Entry informationi

    Entry nameiBIOB_HAHCH
    AccessioniPrimary (citable) accession number: Q2SBD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3