Q2SA17 (TYSY_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	HCH_05857

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella › Hahella chejuensis

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00008
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP-Rule MF_00008		PRO_1000000608
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SA17 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 5ABBCC3A974DB939	FASTA	264	30,587
	10 20 30 40 50 60 MKQYLDMMRH VREQGAVKTD RTGTGTRSVF GYQMRFDLQQ GFPLVTTKKL HLRSIIYELL 70 80 90 100 110 120 WFLNGDTNIK YLKDNGVSIW DEWADESGDL GPVYGYQWRS WPAPNGEHID QISNVLAQIK 130 140 150 160 170 180 RNPDSRRHMV VAYNPAFVDQ MALPPCHAMF QFYVAEGRLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLTHMFAQ QCDLDVGDFI WTGGDVHLYN NHLDQAKEQL DREPRPLPQL QIRRKPASLF 250 260 DYEFEDFEIT GYDPHPHIKA PVAV

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KCTC 2396.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC32507.1.
RefSeq	YP_436932.1. NC_007645.1.
3D structure databases
ProteinModelPortal	Q2SA17.
SMR	Q2SA17. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	349521.HCH_05857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3843465.
KEGG	hch:HCH_05857.
PATRIC	22091934. VBIHahChe29232_5312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	CLSK2527214.
Enzyme and pathway databases
BioCyc	HCHE349521:GHAL-5648-MONOMER.
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_HAHCH

Accession

Primary (citable) accession number: Q2SA17

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	May 29, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program