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Q2S9T9

- SYI_HAHCH

UniProt

Q2S9T9 - SYI_HAHCH

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Protein
Isoleucine--tRNA ligase
Gene
ileS, HCH_05933
Organism
Hahella chejuensis (strain KCTC 2396)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity
Metal bindingi927 – 9271Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHCHE349521:GHAL-5730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HCH_05933
OrganismiHahella chejuensis (strain KCTC 2396)
Taxonomic identifieri349521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella
ProteomesiUP000000238: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022075Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi349521.HCH_05933.

Structurei

3D structure databases

ProteinModelPortaliQ2S9T9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2S9T9-1 [UniParc]FASTAAdd to Basket

« Hide

MSDYKHTLNL PQTDFPMRGN LPQREPDMLK RWGDIELYEK IREEFKGREK    50
FILHDGPPYA NGNIHLGHAV NKILKDIIVK SKTVAGYDAP YVPGWDCHGL 100
PIEHKVETMI GRAGDKVGYK EFRRKCREYA LEQVAKQRED FIRLGVFGDW 150
FKPYLTLDFK TEADIIRALG KIATSGHLQK GYKPVYWSVV GGSALAEAEV 200
EYKDKTSNSI DVSYPAENEQ DLLSAFADAS GEGAVSIVIW TTTPWTLPAS 250
LAVSLGAEID YALTQCSVDG RPQRWIVAEK MLESVMARCG VEDYQVVGRC 300
TGQDLEGKTF LHPFYKRSIP ALLGDHVTLD AGTGVVHTAP DHGMEDFAVC 350
NKYGIETINP LDDRGVYRDN VELFAGEHVY KVDDHVIEVL KERGRLLSHG 400
KITHSYAHCW RTKTPLIYRA TPQWFISMDK QGLRDQALAE IKLVRWVPSW 450
GQNRIEAMIE QSPDWCISRQ RTWGVPIAFF VHKETQELHP DTARLVEEVA 500
KQVEKTGIDA WWDINAEDLL GADAQNYEKV TDTLDVWFDS GVTHSAVLEQ 550
REELGQFPAD LYLEGSDQHR GWFQSSLKTA VAIKGKAPYR QVLTHGFTVD 600
EKGHKMSKSL GNGIEPQEVM NKLGADILRL WVAATDYSAE MVLSKNILER 650
TADSYRRIRN TSRFLLANIN DFDPVKDMVA MDDLLALDRW IVDRAWLLQE 700
ELKKAYEQYN FVQVYQKVHN FCSVELGSFY LDVIKDRQYT MKLDSLGCRS 750
AQTAQYHVIE ALVRWIAPIL SFTAEEIWSY IPGQRAESIF LETFYEGLQP 800
LAEGSEMGRD YWSRLLRVRT SVNKALEEAR NAGKVKGSLT TEVSLFATPE 850
MQHDLNALGE ELRFVFITSG ASVFGVEDAS ESNSVATETE GLRVGIRSSE 900
HKKCGRCWHH REDVGAHGSH EDLCGRCIEN IDGSGEERKY A 941
Length:941
Mass (Da):106,642
Last modified:January 24, 2006 - v1
Checksum:i2C175D4B31DA6BFB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000155 Genomic DNA. Translation: ABC32585.1.
RefSeqiWP_011399643.1. NC_007645.1.
YP_437010.1. NC_007645.1.

Genome annotation databases

EnsemblBacteriaiABC32585; ABC32585; HCH_05933.
GeneIDi3842782.
KEGGihch:HCH_05933.
PATRICi22092096. VBIHahChe29232_5388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000155 Genomic DNA. Translation: ABC32585.1 .
RefSeqi WP_011399643.1. NC_007645.1.
YP_437010.1. NC_007645.1.

3D structure databases

ProteinModelPortali Q2S9T9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349521.HCH_05933.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC32585 ; ABC32585 ; HCH_05933 .
GeneIDi 3842782.
KEGGi hch:HCH_05933.
PATRICi 22092096. VBIHahChe29232_5388.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HCHE349521:GHAL-5730-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KCTC 2396.

Entry informationi

Entry nameiSYI_HAHCH
AccessioniPrimary (citable) accession number: Q2S9T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi