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Q2S8L4 (TRMB_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HCH_06365
OrganismHahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]
Taxonomic identifier349521 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence ABC33010.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288155

Regions

Region139 – 1446Interaction with RNA Potential
Region206 – 2094Substrate binding By similarity

Sites

Active site1331 By similarity
Binding site581S-adenosyl-L-methionine By similarity
Binding site831S-adenosyl-L-methionine By similarity
Binding site1101S-adenosyl-L-methionine By similarity
Binding site1331S-adenosyl-L-methionine By similarity
Binding site1371Substrate By similarity
Binding site1691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2S8L4 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: D1CF33DEC2FC98FD

FASTA22826,087
        10         20         30         40         50         60 
MDNQHLRPIR SFVIRGGRMT ASQQKAFDRL WPEKGLAVEN GLLDLESVFG RRSTVVLEIG 

        70         80         90        100        110        120 
FGMGQSLAEM AASEPDADFI GVEVHKPGVG ALLRNMEEQS LENIRIFWDD ANEVIDKCLP 

       130        140        150        160        170        180 
DASLDRVQLF FPDPWHKKRH NKRRIVQLEF VQRIRAKLKS GGVFHMATDW EPYAEHMMEI 

       190        200        210        220 
MSEAPGMENI SGAGQFSPRP DTRPVTKFEK RGERLGHGVW DLMFRRVD 

« Hide

References

[1]"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent."
Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F.
Nucleic Acids Res. 33:7066-7073(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KCTC 2396.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000155 Genomic DNA. Translation: ABC33010.1. Different initiation.
RefSeqYP_437435.1. NC_007645.1.

3D structure databases

ProteinModelPortalQ2S8L4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349521.HCH_06365.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC33010; ABC33010; HCH_06365.
GeneID3838402.
KEGGhch:HCH_06365.
PATRIC22092928. VBIHahChe29232_5793.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHCHE349521:GHAL-6165-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HAHCH
AccessionPrimary (citable) accession number: Q2S8L4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: June 11, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways