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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Hahella chejuensis (strain KCTC 2396)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58S-adenosyl-L-methionineUniRule annotation1
Binding sitei83S-adenosyl-L-methionineUniRule annotation1
Binding sitei110S-adenosyl-L-methionineUniRule annotation1
Active sitei133By similarity1
Binding sitei133S-adenosyl-L-methionineUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Binding sitei169SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:HCH_06365
OrganismiHahella chejuensis (strain KCTC 2396)
Taxonomic identifieri349521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHahellaceaeHahella
Proteomesi
  • UP000000238 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881551 – 228tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST228

Interactioni

Protein-protein interaction databases

STRINGi349521.HCH_06365.

Structurei

3D structure databases

ProteinModelPortaliQ2S8L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 144Interaction with RNAUniRule annotation6
Regioni206 – 209Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2S8L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNQHLRPIR SFVIRGGRMT ASQQKAFDRL WPEKGLAVEN GLLDLESVFG
60 70 80 90 100
RRSTVVLEIG FGMGQSLAEM AASEPDADFI GVEVHKPGVG ALLRNMEEQS
110 120 130 140 150
LENIRIFWDD ANEVIDKCLP DASLDRVQLF FPDPWHKKRH NKRRIVQLEF
160 170 180 190 200
VQRIRAKLKS GGVFHMATDW EPYAEHMMEI MSEAPGMENI SGAGQFSPRP
210 220
DTRPVTKFEK RGERLGHGVW DLMFRRVD
Length:228
Mass (Da):26,087
Last modified:May 29, 2007 - v2
Checksum:iD1CF33DEC2FC98FD
GO

Sequence cautioni

The sequence ABC33010 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA. Translation: ABC33010.1. Different initiation.
RefSeqiWP_041598991.1. NC_007645.1.

Genome annotation databases

EnsemblBacteriaiABC33010; ABC33010; HCH_06365.
KEGGihch:HCH_06365.
PATRICi22092928. VBIHahChe29232_5793.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000155 Genomic DNA. Translation: ABC33010.1. Different initiation.
RefSeqiWP_041598991.1. NC_007645.1.

3D structure databases

ProteinModelPortaliQ2S8L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349521.HCH_06365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC33010; ABC33010; HCH_06365.
KEGGihch:HCH_06365.
PATRICi22092928. VBIHahChe29232_5793.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_HAHCH
AccessioniPrimary (citable) accession number: Q2S8L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.