ID   GSH1_HAHCH              Reviewed;         525 AA.
AC   Q2S8F7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=Glutamate--cysteine ligase;
DE            EC=6.3.2.2;
DE   AltName: Full=Gamma-glutamylcysteine synthetase;
DE   AltName: Full=Gamma-ECS;
DE            Short=GCS;
GN   Name=gshA; OrderedLocusNames=HCH_06422;
OS   Hahella chejuensis (strain KCTC 2396).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Hahellaceae; Hahella.
OX   NCBI_TaxID=349521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16352867; DOI=10.1093/nar/gki1016;
RA   Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H.,
RA   Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H.,
RA   Park H.-S., Lee H.K., Oh T.K., Kim J.F.;
RT   "Genomic blueprint of Hahella chejuensis, a marine microbe producing
RT   an algicidal agent.";
RL   Nucleic Acids Res. 33:7066-7073(2005).
CC   -!- CATALYTIC ACTIVITY: ATP + L-glutamate + L-cysteine = ADP +
CC       phosphate + gamma-L-glutamyl-L-cysteine.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 1/2.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1
CC       family. Type 1 subfamily.
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DR   EMBL; CP000155; ABC33067.1; -; Genomic_DNA.
DR   RefSeq; YP_437492.1; -.
DR   GeneID; 3843830; -.
DR   GenomeReviews; CP000155_GR; HCH_06422.
DR   KEGG; hch:HCH_06422; -.
DR   NMPDR; fig|349521.5.peg.5629; -.
DR   HOGENOM; Q2S8F7; -.
DR   OMA; Q2S8F7; DTLYLPY.
DR   BioCyc; HCHE349521:HCH_06422-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:HAMAP.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00578; -; 1.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Nucleotide-binding.
FT   CHAIN         1    525       Glutamate--cysteine ligase.
FT                                /FTId=PRO_1000025173.
SQ   SEQUENCE   525 AA;  59532 MW;  90E10169B68FCB67 CRC64;
     MSEKLAARLE KLRRRHPQAL ADIYRGIEKE GLRVDSKGFI AQSDHPQALG SALTHPNITT
     DYSEALLELI TPVTREVDEL LTSLQEIHQF VHANLPAGES LWAGSMPSLL DGDESIRIAE
     YGESNLGKIK HVYRRGLAYR YGRIMQSIAG VHFNFSLGDD FWRAYQEVLA QSAPLQEFKS
     ESYFSLIRNF RRWSWLLMYL FGASPALDRS FLNGRDHKLD QFGTDTLGLP HATSLRMSDL
     GYQNNAQSSL KICFNHLSTY VKTLYDATHT PFPRYEAIGL QRDGEYIQLN ANLLQIENEY
     YNTIRPKRVT QSGEKPIQAL KRRGIEYIEV RCLDLDPFSP IGVSESQIRF LDAFLLTCLL
     SDSAKIVDEE CSIIEENFLT AVSRGRATDV ELVRLLQNDY VQGGLQEWAS RILEQVELCA
     RELDAIKGGD SYAVAVRDAR AKVNDPSLTP SARTYAAVSN GQSYVDWTLA MSQAHHQTLI
     ANPLSPERMQ HYVRAGEQSW ADERALREAD NLSFDAYLKQ YLTYV
//