Q2S8F7 (GSH1_HAHCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	HCH_06422

Organism

Hahella chejuensis (strain KCTC 2396) [Complete proteome] [HAMAP]

Taxonomic identifier

349521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Hahellaceae › Hahella

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025173

Sequences

	Sequence		Length	Mass (Da)
	Q2S8F7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 90E10169B68FCB67	FASTA	525	59,532
	10 20 30 40 50 60 MSEKLAARLE KLRRRHPQAL ADIYRGIEKE GLRVDSKGFI AQSDHPQALG SALTHPNITT 70 80 90 100 110 120 DYSEALLELI TPVTREVDEL LTSLQEIHQF VHANLPAGES LWAGSMPSLL DGDESIRIAE 130 140 150 160 170 180 YGESNLGKIK HVYRRGLAYR YGRIMQSIAG VHFNFSLGDD FWRAYQEVLA QSAPLQEFKS 190 200 210 220 230 240 ESYFSLIRNF RRWSWLLMYL FGASPALDRS FLNGRDHKLD QFGTDTLGLP HATSLRMSDL 250 260 270 280 290 300 GYQNNAQSSL KICFNHLSTY VKTLYDATHT PFPRYEAIGL QRDGEYIQLN ANLLQIENEY 310 320 330 340 350 360 YNTIRPKRVT QSGEKPIQAL KRRGIEYIEV RCLDLDPFSP IGVSESQIRF LDAFLLTCLL 370 380 390 400 410 420 SDSAKIVDEE CSIIEENFLT AVSRGRATDV ELVRLLQNDY VQGGLQEWAS RILEQVELCA 430 440 450 460 470 480 RELDAIKGGD SYAVAVRDAR AKVNDPSLTP SARTYAAVSN GQSYVDWTLA MSQAHHQTLI 490 500 510 520 ANPLSPERMQ HYVRAGEQSW ADERALREAD NLSFDAYLKQ YLTYV

References

[1]

"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent." Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G., Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K., Oh T.K., Kim J.F. Nucleic Acids Res. 33:7066-7073(2005) [PubMed: 16352867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KCTC 2396.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000155 Genomic DNA. Translation: ABC33067.1.
RefSeq	YP_437492.1. NC_007645.1.
3D structure databases
ProteinModelPortal	Q2S8F7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2S8F7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3843830.
GenomeReviews	Gene locus HCH_06422 in contig CP000155_GR.
KEGG	hch:HCH_06422.
NMPDR	fig|349521.5.peg.5629.
PATRIC	22093034. VBIHahChe29232_5846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2918.
HOGENOM	HBG289023.
OMA	ADTLYLP.
PhylomeDB	Q2S8F7.
ProtClustDB	PRK02107.
Enzyme and pathway databases
BioCyc	HCHE349521:HCH_06422-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_HAHCH

Accession

Primary (citable) accession number: Q2S8F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program