Reviewed, UniProtKB/Swiss-Prot Q2S5G4 (CYSH_SALRD)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	SRU_0422

Organism

Salinibacter ruber (strain DSM 13855) [Complete proteome] [HAMAP]

Taxonomic identifier

309807 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteria › Sphingobacteriales › Rhodothermaceae › Salinibacter

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite By similarity.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Phosphoadenosine phosphosulfate reductase		PRO_1000075076

Sequences

	Sequence		Length	Mass (Da)
	Q2S5G4-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 2007E8A7AB199D84	FASTA	252	28,593
	10 20 30 40 50 60 MAFASDESAS PWSSTRLAAL NAQFEPHGPK AILNWATHTF GDDLAQGTGF GPSGIVIMHM 70 80 90 100 110 120 LADLRPGTTV FYLDTDLLFP ETYELCDDLD ERLDVDVTRV HGGLSLDEQA EQEGEELWNR 130 140 150 160 170 180 NPNRCCFLRK VKPLRNFLDD RRAWITGVRR DQSERRADTD ILSWEGQYGV FKINPLANWT 190 200 210 220 230 240 QKEVWKYLFE HDLPYNPKHD QGYPSLGCVP CTEPVDQADG YSREGRWSDR DKTECGLHTS 250 PEDEDGAHAA ES

References

[1]

"The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea." Mongodin E.F., Nelson K.E., Daugherty S., DeBoy R.T., Wister J., Khouri H., Weidman J., Walsh D.A., Papke R.T., Sanchez Perez G., Sharma A.K., Nesbo C.L., MacLeod D., Bapteste E., Doolittle W.F., Charlebois R.L., Legault B., Rodriguez-Valera F. Proc. Natl. Acad. Sci. U.S.A. 102:18147-18152(2005) [PubMed: 16330755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000159 Genomic DNA. Translation: ABC44343.1.
RefSeq	YP_444567.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3852402.
GenomeReviews	Gene locus SRU_0422 in contig CP000159_GR.
KEGG	sru:SRU_0422.
NMPDR	fig|309807.5.peg.387.
TIGR	SRU_0422.
Phylogenomic databases
HOGENOM	Q2S5G4.
Enzyme and pathway databases
BioCyc	SRUB309807:SRU_0422-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_SALRD

Accession

Primary (citable) accession number: Q2S5G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 24, 2006
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)