Q2S567 (PURQ_SALRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	SRU_0521

Organism

Salinibacter ruber (strain DSM 13855 / M31) [Reference proteome] [HAMAP]

Taxonomic identifier

309807 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidetes Order II. Incertae sedis › Rhodothermaceae › Salinibacter › Salinibacter ruber

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00421.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Phosphoribosylformylglycinamidine synthase 1 HAMAP-Rule MF_00421		PRO_0000252729
Regions
	Domain	5 – 235	231	Glutamine amidotransferase type-1
Sites
	Active site	88	1	Nucleophile By similarity
	Active site	205	1	By similarity
	Active site	207	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2S567 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: D385CC7DDCB51A30	FASTA	235	25,198
	10 20 30 40 50 60 MSARFGVVVF PGSNCDHDAY HAAHDVFDQE ARFIWHEEAS LGDVDVVIVP GGFSYGDYLR 70 80 90 100 110 120 SGAVARFSPI MQDVVRFAED GGLVFGICNG FQVLCEAGLL PGTLMRNESL RFVCKDTPLR 130 140 150 160 170 180 VENAGTPFTN ALTEGQVITI PVSHGEGRYY ADEDVLARIE ANDQVLLRYS TADGAVTDEA 190 200 210 220 230 NPNGSVHGIA GLVNEAGNVC GLMPHPERCV ESLLGGDDGR LIFESLLNHV SIVAA

References

[1]

"The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea." Mongodin E.F., Nelson K.E., Daugherty S., DeBoy R.T., Wister J., Khouri H., Weidman J., Walsh D.A., Papke R.T., Sanchez Perez G., Sharma A.K., Nesbo C.L., MacLeod D., Bapteste E., Doolittle W.F., Charlebois R.L., Legault B., Rodriguez-Valera F. Proc. Natl. Acad. Sci. U.S.A. 102:18147-18152(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13855 / M31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000159 Genomic DNA. Translation: ABC43823.1.
RefSeq	YP_444664.1. NC_007677.1.
3D structure databases
ProteinModelPortal	Q2S567.
ModBase	Search...
Protein-protein interaction databases
STRING	309807.SRU_0521.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC43823; ABC43823; SRU_0521.
GeneID	3851519.
KEGG	sru:SRU_0521.
PATRIC	23423387. VBISalRub86502_0543.
Phylogenomic databases
eggNOG	COG0047.
HOGENOM	HOG000238240.
KO	K01952.
OMA	FPGTNCD.
PhylomeDB	Q2S567.
ProtClustDB	CLSK2773662.
Enzyme and pathway databases
BioCyc	SRUB309807:GJJD-520-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00421. PurQ.
InterPro	IPR017926. GATASE. IPR010075. PRibForGlyAmidine_synth_I. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_SALRD

Accession

Primary (citable) accession number: Q2S567

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	January 24, 2006
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program