Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q2S4I8

- BIOB_SALRD

UniProt

Q2S4I8 - BIOB_SALRD

Protein

Biotin synthase

Gene

bioB

Organism
Salinibacter ruber (strain DSM 13855 / M31)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi109 – 1091Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi271 – 2711Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSRUB309807:GJJD-754-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:SRU_0756
    OrganismiSalinibacter ruber (strain DSM 13855 / M31)
    Taxonomic identifieri309807 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeSalinibacter
    ProteomesiUP000008674: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Biotin synthasePRO_0000381607Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi309807.SRU_0756.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2S4I8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiTCENTLR.
    OrthoDBiEOG622PMP.
    PhylomeDBiQ2S4I8.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2S4I8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAWRTLATQ VLNGTPLSSS QALDVVHADD DEVLALLDAA FRVRRHHHGR    50
    RVRIHVLQNA KSGVCPEDCA FCSQSLKFDS DPEQYGMQQV DQIVEGAKAA 100
    WDKGAVTYCI VTATRGPHSS EVDVVCEATR RIKETYPMDV CASLGLLDAK 150
    QARKLADAGV DRYNHNLETS CDHFGNVVTT HEWSDRVETV KQAKAAGMEA 200
    CCGGIIGLGE ARADWVDLAL ALREIGVESV PVNFLNPRSG TPLEDVDTVR 250
    PQDCLKALAM FRLVHPEADL RMAGGREVVL DQMQPLALYA ANSFFTDGYL 300
    TTGGQGESKD YRMIQQAGFE PVIVEDGPER QTPATADDTP SGDPEAADRR 350
    RQPSAGPAG 359
    Length:359
    Mass (Da):39,005
    Last modified:January 24, 2006 - v1
    Checksum:i86AE7458EC716DB6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000159 Genomic DNA. Translation: ABC44565.1.
    RefSeqiYP_444893.1. NC_007677.1.

    Genome annotation databases

    EnsemblBacteriaiABC44565; ABC44565; SRU_0756.
    GeneIDi3851287.
    KEGGisru:SRU_0756.
    PATRICi23423857. VBISalRub86502_0778.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000159 Genomic DNA. Translation: ABC44565.1 .
    RefSeqi YP_444893.1. NC_007677.1.

    3D structure databases

    ProteinModelPortali Q2S4I8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 309807.SRU_0756.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC44565 ; ABC44565 ; SRU_0756 .
    GeneIDi 3851287.
    KEGGi sru:SRU_0756.
    PATRICi 23423857. VBISalRub86502_0778.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi TCENTLR.
    OrthoDBi EOG622PMP.
    PhylomeDBi Q2S4I8.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci SRUB309807:GJJD-754-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13855 / M31.

    Entry informationi

    Entry nameiBIOB_SALRD
    AccessioniPrimary (citable) accession number: Q2S4I8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3