Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2S415 (SYL_SALRD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SRU_0933
OrganismSalinibacter ruber (strain DSM 13855 / M31) [Reference proteome] [HAMAP]
Taxonomic identifier309807 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeSalinibacter

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009423

Regions

Motif42 – 5312"HIGH" region HAMAP-Rule MF_00049
Motif635 – 6395"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6381ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2S415 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: DE2DC62AD14854EA

FASTA86398,948
        10         20         30         40         50         60 
MAYPFHDIET KWQQYWEEHQ TFRTPDEVPD DQEEFYVLDM FPYPSGSGLH VGHPEGYTAT 

        70         80         90        100        110        120 
DIVARYKRKQ GFNVLHPMGW DAFGLPAEQY ALKTNTHPRE TTEKNIAQFK RQLKRLGFSY 

       130        140        150        160        170        180 
DWQREINTTD PDYYKWTQWI FLQLYEKGLA YQSEEPVWWC EELGTVLANE EVIDGKSERG 

       190        200        210        220        230        240 
GYPCERVPMR QWVLKITEYA DRLLEGLEDL DWPESTKEMQ RNWIGRSEGA NVYFDLVGAD 

       250        260        270        280        290        300 
DALEVYTTRP DTLFGATYMV LAPEHELLDE ITTDEHREDV DEYCRQALRK SERKRQQQGD 

       310        320        330        340        350        360 
KTGVFTGGYA VNPVNGEEIP IWVADYVLVS YGTGAIMAVP AHDERDHAFA NKYDLPIREV 

       370        380        390        400        410        420 
VEGGDIDEEA YTGDGPHVHS ANEAVSLNGL RNEEAKEAIT EWLDEEEKGE RTVNYQLQDW 

       430        440        450        460        470        480 
LFSRQRYWGE PFPIVFTEDG EDKPVPEEEL PVTLPDLDVF EPSGTPEGPL ATIEDWRETT 

       490        500        510        520        530        540 
DPETGEPAQR ETNTMPQWAG SCWYYLRFID PDNDEQLVDP EKEEYWMPVD LYVGGSEHAV 

       550        560        570        580        590        600 
LHLLYARFWH KVLYDAGVVS TKEPFQTLVH QGMILGETEY TAYRDDAGEF VSAEQVDDDA 

       610        620        630        640        650        660 
DLTPVPVDDG DVKKDGDVFV LADRPAVRVD ARSHKMSKSR GNVINPDDVV DEYGADTLRL 

       670        680        690        700        710        720 
YEMFMGPLEQ DKPWSTDDME GVHRFLNRIW RLVVDADSGG LAVSDEEPDR EQLRTLHRTI 

       730        740        750        760        770        780 
KTVTEDIEAR DFNTAIAAMM EFVNAANKWD ALPRQVATPF VLLLSPFAPH LAEELWARLG 

       790        800        810        820        830        840 
HDQSLAYADW PAYDDELIRR EVVEMPVQVD GTVRATIEVA ADAEEADVLA TAKEAENVAR 

       850        860 
HLDDEDLQRE IYVPGQIVNF VTG 

« Hide

References

[1]"The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea."
Mongodin E.F., Nelson K.E., Daugherty S., DeBoy R.T., Wister J., Khouri H., Weidman J., Walsh D.A., Papke R.T., Sanchez Perez G., Sharma A.K., Nesbo C.L., MacLeod D., Bapteste E., Doolittle W.F., Charlebois R.L., Legault B., Rodriguez-Valera F.
Proc. Natl. Acad. Sci. U.S.A. 102:18147-18152(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13855 / M31.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000159 Genomic DNA. Translation: ABC46207.1.
RefSeqYP_445066.1. NC_007677.1.

3D structure databases

ProteinModelPortalQ2S415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309807.SRU_0933.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC46207; ABC46207; SRU_0933.
GeneID3850817.
KEGGsru:SRU_0933.
PATRIC23424243. VBISalRub86502_0967.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
PhylomeDBQ2S415.
ProtClustDBCLSK2775249.

Enzyme and pathway databases

BioCycSRUB309807:GJJD-931-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_SALRD
AccessionPrimary (citable) accession number: Q2S415
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries