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Protein

Malate dehydrogenase

Gene

mdh

Organism
Salinibacter ruber (strain DSM 13855 / M31)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32NADUniRule annotation1
Binding sitei81SubstrateUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei94NADUniRule annotation1
Binding sitei119SubstrateUniRule annotation1
Binding sitei150SubstrateUniRule annotation1
Active sitei174Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADUniRule annotation6
Nucleotide bindingi117 – 119NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:SRU_1571
OrganismiSalinibacter ruber (strain DSM 13855 / M31)
Taxonomic identifieri309807 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeSalinibacter
Proteomesi
  • UP000008674 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002419671 – 314Malate dehydrogenaseAdd BLAST314

Interactioni

Protein-protein interaction databases

STRINGi309807.SRU_1571.

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 22Combined sources13
Beta strandi25 – 31Combined sources7
Helixi37 – 52Combined sources16
Beta strandi57 – 64Combined sources8
Helixi65 – 67Combined sources3
Beta strandi71 – 75Combined sources5
Helixi88 – 106Combined sources19
Beta strandi113 – 116Combined sources4
Helixi121 – 132Combined sources12
Helixi136 – 138Combined sources3
Beta strandi139 – 141Combined sources3
Helixi144 – 159Combined sources16
Helixi163 – 165Combined sources3
Beta strandi166 – 174Combined sources9
Helixi175 – 177Combined sources3
Beta strandi178 – 187Combined sources10
Helixi192 – 194Combined sources3
Helixi198 – 209Combined sources12
Helixi211 – 219Combined sources9
Helixi225 – 240Combined sources16
Beta strandi244 – 254Combined sources11
Helixi255 – 257Combined sources3
Beta strandi259 – 270Combined sources12
Beta strandi273 – 277Combined sources5
Helixi284 – 309Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NEPX-ray1.55X1-314[»]
ProteinModelPortaliQ2S289.
SMRiQ2S289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiSARCRYE.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2S289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTVIGAGN VGATVAECVA RQDVAKEVVM VDIKDGMPQG KALDMRESSP
60 70 80 90 100
IHGFDTRVTG TNDYGPTEDS DVCIITAGLP RSPGMSRDDL LAKNTEIVGG
110 120 130 140 150
VTEQFVEGSP DSTIIVVANP LDVMTYVAYE ASGFPTNRVM GMAGVLDTGR
160 170 180 190 200
FRSFIAEELD VSVRDVQALL MGGHGDTMVP LPRYTTVGGI PVPQLIDDAR
210 220 230 240 250
IEEIVERTKG AGGEIVDLMG TSAWYAPGAA AAEMTEAILK DNKRILPCAA
260 270 280 290 300
YCDGEYGLDD LFIGVPVKLG AGGVEEVIEV DLDADEKAQL KTSAGHVHSN
310
LDDLQRLRDE GKIG
Length:314
Mass (Da):33,256
Last modified:January 24, 2006 - v1
Checksum:iFD2F7BDA3F8249E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000159 Genomic DNA. Translation: ABC45135.1.
RefSeqiWP_011404318.1. NC_007677.1.
YP_445692.1. NC_007677.1.

Genome annotation databases

EnsemblBacteriaiABC45135; ABC45135; SRU_1571.
GeneIDi3850691.
KEGGisru:SRU_1571.
PATRICi23425577. VBISalRub86502_1625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000159 Genomic DNA. Translation: ABC45135.1.
RefSeqiWP_011404318.1. NC_007677.1.
YP_445692.1. NC_007677.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NEPX-ray1.55X1-314[»]
ProteinModelPortaliQ2S289.
SMRiQ2S289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309807.SRU_1571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC45135; ABC45135; SRU_1571.
GeneIDi3850691.
KEGGisru:SRU_1571.
PATRICi23425577. VBISalRub86502_1625.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiSARCRYE.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_SALRD
AccessioniPrimary (citable) accession number: Q2S289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.