Q2S160 (Q2S160_SALRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116 EMBL ABC46119.1
Ordered Locus Names:	SRU_1961

Organism

Salinibacter ruber (strain DSM 13855 / M31)

Taxonomic identifier

309807 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidetes Order II. Incertae sedis › Rhodothermaceae › Salinibacter

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116 SAAS SAAS008181
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116 SAAS SAAS008181
Cofactor	Magnesium By similarity. HAMAP MF_00116 SAAS SAAS008181
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116 SAAS SAAS008181
Ligand	Magnesium HAMAP MF_00116 SAAS SAAS008181 Metal-binding HAMAP MF_00116 SAAS SAAS008181
Molecular function	Hydrolase HAMAP MF_00116 SAAS SAAS008181 EMBL ABC46119.1
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	69 – 71	3	Substrate binding By similarity HAMAP MF_00116
	Region	86 – 88	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	82	1	Substrate By similarity HAMAP MF_00116
	Binding site	96	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	Q2S160 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 6002CB9EE8B28895	FASTA	150	15,795
	10 20 30 40 50 60 MIDVSITRLP HAEGLALPSY ETSASAGMDL RAAVPADAPV VLDPGARALV DTGLQLALPE 70 80 90 100 110 120 GHEGQVRPRS GLAHDHGVTV LNSPGTIDAD YRGEVKVLLV NHGAEPFTIE RGMRIAQLVV 130 140 150 ARHARVEWVH REALEETVRG PGGFGSTGTA

References

[1]

"The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea." Mongodin E.F., Nelson K.E., Daugherty S., DeBoy R.T., Wister J., Khouri H., Weidman J., Walsh D.A., Papke R.T., Sanchez Perez G., Sharma A.K., Nesbo C.L., MacLeod D., Bapteste E., Doolittle W.F., Charlebois R.L., Legault B., Rodriguez-Valera F. Proc. Natl. Acad. Sci. U.S.A. 102:18147-18152(2005) [PubMed: 16330755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13855 / M31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000159 Genomic DNA. Translation: ABC46119.1.
RefSeq	YP_446071.1. NC_007677.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2S160.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3853142.
GenomeReviews	Gene locus SRU_1961 in contig CP000159_GR.
KEGG	sru:SRU_1961.
NMPDR	fig|309807.5.peg.1856.
PATRIC	23426417. VBISalRub86502_2034.
TIGR	SRU_1961.
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	LDLRACI.
PhylomeDB	Q2S160.
ProtClustDB	CLSK2775198.
Enzyme and pathway databases
BioCyc	SRUB309807:SRU_1961-MONOMER.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2S160_SALRD

Accession

Primary (citable) accession number: Q2S160

Entry history

Integrated into UniProtKB/TrEMBL:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)