Q2S008 (PCKA2_SALRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP] 2
Short name=PEP carboxykinase 2
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase 2

Gene names

Name:	pckA2
Ordered Locus Names:	SRU_2373

Organism

Salinibacter ruber (strain DSM 13855 / M31)

Taxonomic identifier

309807 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidetes Order II. Incertae sedis › Rhodothermaceae › Salinibacter

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity HAMAP MF_00453.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Phosphoenolpyruvate carboxykinase [ATP] 2 HAMAP MF_00453		PRO_0000236939
Regions
	Nucleotide binding	232 – 239	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2S008 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 094AEDCD6E1F719B	FASTA	530	59,057
	10 20 30 40 50 60 MSDLDLSQYG ITPDTTLRNA DPARLYEEAI HYDPTAAIAH SGALTIRSGE KTGRSPADKR 70 80 90 100 110 120 IVRHPNSEDD IWWGPINMEI DDHTFEINKE RAQDYLNTRQ RIYVMDGFAG WDPAHRLKVR 130 140 150 160 170 180 IICSRPYHAL FMHNMLIRPS QEELASFGEP DFVIYNAGEF PANRQTKHMS SKTSVDLSFE 190 200 210 220 230 240 NQEMVILGTE YAGEMKKGVF TVMHYLMPKK DVLSMHCSAN EGDEGDVSLF FGLSGTGKTT 250 260 270 280 290 300 LSADPNRKLI GDDEHCWSDD GVFNIEGGCY AKAVGLSEEE EPEIYNAIRY GTVLENMVYD 310 320 330 340 350 360 EDTRAVDYDD TSITQNTRAS YPLDYIDRAK IPGMGGHPDN IIFLTYDAFG VMPPVSKLTP 370 380 390 400 410 420 EQAMYHFISG YTAKVAGTEV GVDEPQATFS ACFGAAFLVW PPDKYAEMLA EKIRAHDAEA 430 440 450 460 470 480 WLVNTGITGG PYGVGHRVPL EHTRAMIDAI HDGSLLDAPK KTEPVFGLDV PTECPNVPND 490 500 510 520 530 ILMPRETWDD PQAYDEKAEH LVGLFHDHFE KYEDEAAPAI AEAGPQLQAA

References

[1]

"The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea." Mongodin E.F., Nelson K.E., Daugherty S., DeBoy R.T., Wister J., Khouri H., Weidman J., Walsh D.A., Papke R.T., Sanchez Perez G., Sharma A.K., Nesbo C.L., MacLeod D., Bapteste E., Doolittle W.F., Charlebois R.L., Legault B., Rodriguez-Valera F. Proc. Natl. Acad. Sci. U.S.A. 102:18147-18152(2005) [PubMed: 16330755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13855 / M31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000159 Genomic DNA. Translation: ABC44838.1.
RefSeq	YP_446473.1. NC_007677.1.
3D structure databases
ProteinModelPortal	Q2S008.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2S008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3852121.
GenomeReviews	Gene locus SRU_2373 in contig CP000159_GR.
KEGG	sru:SRU_2373.
NMPDR	fig|309807.5.peg.2245.
PATRIC	23427305. VBISalRub86502_2472.
TIGR	SRU_2373.
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HBG293387.
OMA	VYNPSYE.
PhylomeDB	Q2S008.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	SRUB309807:SRU_2373-MONOMER.
Family and domain databases
HAMAP	MF_00453. PEPCK_ATP. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KO	K01610.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. PckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA2_SALRD

Accession

Primary (citable) accession number: Q2S008

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program