Q2RXS7 (ISPE_RHORT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Rru_A0263

Organism

Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255)

Taxonomic identifier

269796 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235125
Regions
	Nucleotide binding	103 – 113	11	ATP Potential
Sites
	Active site	18	1	By similarity
	Active site	145	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RXS7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 4D549205F6E87101	FASTA	304	31,620
	10 20 30 40 50 60 MTAVFPAADS VIEAACAKVN LTLHVTGRRA NGYHDLDSLV VFSGAHDTLI ACPAEGLSLA 70 80 90 100 110 120 MTGPTAALLD AEDDNIILRA ARRLAERAGH RPDARLTLIK RLPVAAGIGG GSADGAATLR 130 140 150 160 170 180 ALSRLWKIAL PQAEMLTLAA ELGADVPVCL VGKAVTMAGI GDHLTPAPPL PEAWLLLVNP 190 200 210 220 230 240 RRAVPTPQVF KARQGGFSAA NPLTESPASA QALAEALKRR TNDLAEPARR IEPVIDEVLR 250 260 270 280 290 300 TLAALPGCLL ARMSGSGATC FGLFADQTSA DFGMASLRET HGSWWASADR LIGDTARVPA ALAV

References

[1]

"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11170 / NCIB 8255.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000230 Genomic DNA. Translation: ABC21068.1.
RefSeq	YP_425355.1. NC_007643.1.
3D structure databases
ProteinModelPortal	Q2RXS7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RXS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3834583.
GenomeReviews	Gene locus Rru_A0263 in contig CP000230_GR.
KEGG	rru:Rru_A0263.
PATRIC	23324091. VBIRhoRub82919_0317.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	GPFAKGL.
PhylomeDB	Q2RXS7.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	RRUB269796:RRU_A0263-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_RHORT

Accession

Primary (citable) accession number: Q2RXS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program