Q2RX18 (CHEB1_RHORT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Rru_A0523

Organism

Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255)

Taxonomic identifier

269796 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264311
Regions
	Domain	18 – 136	119	Response regulatory
	Domain	190 – 387	198	CheB-type methylesterase
Sites
	Active site	204	1	By similarity
	Active site	233	1	By similarity
	Active site	329	1	By similarity
Amino acid modifications
	Modified residue	69	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RX18 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 4C87E4AB2AF0E16D	FASTA	387	40,793
	10 20 30 40 50 60 MTITTSEATS HTLGGPIRVM VVDDSAVVRG LETRMLEEDP AIQVVASVGN GQMAVQALDR 70 80 90 100 110 120 HDIEVVILDI EMPVMDGLTA LPELLRKSPN LKVIMASTLT LRNAEVTLKA LQMGASECLA 130 140 150 160 170 180 KPTTSREISG GTDFRHDLVE KVKALGGARR RALGRAAPTA RAGGAVVERK VGALPSLMAQ 190 200 210 220 230 240 RAQQPISLRP AAEERPDIIA IGSSTGGPQA LFTVFGDMRK GGWPSQPIVV TQHMPATFTT 250 260 270 280 290 300 ILAGHIERVA GVPTAEAKDG DPIRGGHIYI APGDYHMVVE TRGTEKILRL NQDPPESFCR 310 320 330 340 350 360 PAVDPLFRSV AKAYGRRVLA VVLTGMGADG SKGGKIIAES GGTVIAQDEP SSVVWGMPGA 370 380 TAQIGACSAV LPLKDIAAYV LRSANKR

References

[1]

"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11170 / NCIB 8255.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000230 Genomic DNA. Translation: ABC21327.1.
RefSeq	YP_425614.1. NC_007643.1.
3D structure databases
ProteinModelPortal	Q2RX18.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RX18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3834491.
GenomeReviews	Gene locus Rru_A0523 in contig CP000230_GR.
KEGG	rru:Rru_A0523.
NMPDR	fig|1085.1.peg.1815.
PATRIC	23324621. VBIRhoRub82919_0577.
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	THVIMAS.
PhylomeDB	Q2RX18.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	RRUB269796:RRU_A0523-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RHORT

Accession

Primary (citable) accession number: Q2RX18

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program