Reviewed, UniProtKB/Swiss-Prot Q2RWK3 (SYE1_RHORT)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Rru_A0688

Organism

Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) [Complete proteome] [HAMAP]

Taxonomic identifier

269796 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367751
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RWK3-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 3853C049F04796E6	FASTA	444	48,292
	10 20 30 40 50 60 MSSVLVRFAP SPTGRLHLGN ARVAVLNWLF AHAHGGAMVL RLDDTDIARG TAEFAQAIRD 70 80 90 100 110 120 DLLWLGLTWT REESQLARAA RHAEAVDQLK AAGRLYACYE TPEELEYRRR RQRAQGLPPI 130 140 150 160 170 180 YDRAGLALSA AERAALEAEG RKPHWRFRLD HEETSWLDLA RGPCHAHGGH LSDPVLVRED 190 200 210 220 230 240 GSLLYTLPSV VDDIDFAISH VIRGEDHVVN TAVQIQICRA LGAEPPHYAH LPLVLTAEGG 250 260 270 280 290 300 GLSKRDNALS LGDLRAQGIE PAAINALLAA LGTAEAPDPL KSLDELAQGF RLDAFGRAAP 310 320 330 340 350 360 RLDPADLVRL SARIYHALSP SEARARGIAV SDALWLALRA NLTTLSDLDE LLPVVEGEIT 370 380 390 400 410 420 PLIAEEDRAM IDEAARLLPE TPWDATTWAT WTAAVKTATG RKGKGLFMPL RRALTGVDHG 430 440 PELAALLPLI GRDKALARLR GEKA

References

[1]

"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000230 Genomic DNA. Translation: ABC21492.1.
RefSeq	YP_425779.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RWK3.
Genome annotation databases
GeneID	3834539.
GenomeReviews	Gene locus Rru_A0688 in contig CP000230_GR.
KEGG	rru:Rru_A0688.
NMPDR	fig|1085.1.peg.1656.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2RWK3.
OMA	LRLDDTD.
Enzyme and pathway databases
BioCyc	RRUB269796:RRU_A0688-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RHORT

Accession

Primary (citable) accession number: Q2RWK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 24, 2006
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)