Reviewed, UniProtKB/Swiss-Prot Q2RVX7 (METK1_RHORT)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
    AdoMet synthetase 1
    MAT 1

Gene names

Name:	metK1
Ordered Locus Names:	Rru_A0917

Organism

Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) [Complete proteome] [HAMAP]

Taxonomic identifier

269796 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00086
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	S-adenosylmethionine synthetase 1 HAMAP MF_00086		PRO_0000241030
Regions
	Nucleotide binding	258 – 265	8	ATP Potential
Sites
	Metal binding	17	1	Magnesium By similarity
	Metal binding	43	1	Potassium By similarity
	Metal binding	262	1	Potassium By similarity
	Metal binding	270	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RVX7-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 6FF9276010CF87FA	FASTA	383	40,867
	10 20 30 40 50 60 MRAYCVTSES VTEGHPDKVC DQISDGILDA CLAQDPAARV AVETLVSGNT VFIAGEITTT 70 80 90 100 110 120 ARLDAVRTAR EVIEDIGYAD PALGFDSGSC FILTNLRTQS PDIDLGVSRG AELGAGDQGV 130 140 150 160 170 180 LYGYACDETA SLMPAPIHMA HGLSLRLAAA RHGGVLPWLR PDGKTQVTVR YDGEGRPEAL 190 200 210 220 230 240 TSVIVSTQHD EGVDRETLVR GIIEAVIYPE VGGWLKPETR VLINPTGRFV IGGPAGDTGV 250 260 270 280 290 300 TGRKLMVDTY GGCARHGGGA FSGKDSTKVD RTAAYMARYA AKNVVAAGLA RRCEVALAYA 310 320 330 340 350 360 IGERLPEMVS VETFGTETID VDRLTAAVRE TFPFSVSGMI AALDLRRPIF RKTAAYGHFG 370 380 RENKGFQWEK TDRVDALRAV CGL

References

[1]

"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000230 Genomic DNA. Translation: ABC21718.1.
RefSeq	YP_426005.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RVX7.
Genome annotation databases
GeneID	3833953.
GenomeReviews	Gene locus Rru_A0917 in contig CP000230_GR.
KEGG	rru:Rru_A0917.
NMPDR	fig|1085.1.peg.3455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2RVX7.
OMA	PWEVPKN.
Enzyme and pathway databases
BioCyc	RRUB269796:RRU_A0917-MON.
Family and domain databases
HAMAP	MF_00086. [Tree]
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_RHORT

Accession

Primary (citable) accession number: Q2RVX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	January 24, 2006
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)