ID   Q2RUK0_RHORT            Unreviewed;       519 AA.
AC   Q2RUK0;
DT   24-JAN-2006, integrated into UniProtKB/TrEMBL.
DT   24-JAN-2006, sequence version 1.
DT   27-MAR-2024, entry version 104.
DE   RecName: Full=Nitrogenase protein alpha chain {ECO:0000256|RuleBase:RU004022};
DE            EC=1.18.6.1 {ECO:0000256|RuleBase:RU004022};
GN   OrderedLocusNames=Rru_A1394 {ECO:0000313|EMBL:ABC22195.1};
OS   Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 /
OS   NCIMB 8255 / S1).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Rhodospirillum.
OX   NCBI_TaxID=269796 {ECO:0000313|EMBL:ABC22195.1, ECO:0000313|Proteomes:UP000001929};
RN   [1] {ECO:0000313|EMBL:ABC22195.1, ECO:0000313|Proteomes:UP000001929}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1
RC   {ECO:0000313|Proteomes:UP000001929};
RX   PubMed=21886856; DOI=10.4056/sigs.1804360;
RA   Munk A.C., Copeland A., Lucas S., Lapidus A., Del Rio T.G., Barry K.,
RA   Detter J.C., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D.,
RA   Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Kyrpides N.C.,
RA   Mavromatis K., Richardson P., Rohde M., Goker M., Klenk H.P., Zhang Y.,
RA   Roberts G.P., Reslewic S., Schwartz D.C.;
RT   "Complete genome sequence of Rhodospirillum rubrum type strain (S1).";
RL   Stand. Genomic Sci. 4:293-302(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC         ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC         phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=1.18.6.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000805,
CC         ECO:0000256|RuleBase:RU004022};
CC   -!- COFACTOR:
CC       Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143;
CC         Evidence={ECO:0000256|ARBA:ARBA00001919};
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC       {ECO:0000256|ARBA:ARBA00011515}.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC       {ECO:0000256|ARBA:ARBA00011002, ECO:0000256|RuleBase:RU004021}.
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DR   EMBL; CP000230; ABC22195.1; -; Genomic_DNA.
DR   RefSeq; WP_011389148.1; NC_007643.1.
DR   RefSeq; YP_426482.1; NC_007643.1.
DR   AlphaFoldDB; Q2RUK0; -.
DR   STRING; 269796.Rru_A1394; -.
DR   EnsemblBacteria; ABC22195; ABC22195; Rru_A1394.
DR   KEGG; rru:Rru_A1394; -.
DR   PATRIC; fig|269796.9.peg.1463; -.
DR   eggNOG; COG2710; Bacteria.
DR   HOGENOM; CLU_025876_1_0_5; -.
DR   PhylomeDB; Q2RUK0; -.
DR   Proteomes; UP000001929; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd01977; Nitrogenase_VFe_alpha; 1.
DR   Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 3.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR005974; Nase_asu.
DR   InterPro; IPR010143; Nase_comp1_asu.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR011290; Nase_Fe-Fe_asu.
DR   NCBIfam; TIGR01284; alt_nitrog_alph; 1.
DR   NCBIfam; TIGR01861; ANFD; 1.
DR   NCBIfam; TIGR01862; N2-ase-Ialpha; 1.
DR   PANTHER; PTHR43457; NITROGENASE MOLYBDENUM-IRON PROTEIN ALPHA CHAIN; 1.
DR   PANTHER; PTHR43457:SF1; NITROGENASE MOLYBDENUM-IRON PROTEIN ALPHA CHAIN; 1.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU004022};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|RuleBase:RU004022};
KW   Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW   ECO:0000256|RuleBase:RU004021};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|RuleBase:RU004022};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001929}.
FT   DOMAIN          49..447
FT                   /note="Nitrogenase/oxidoreductase component 1"
FT                   /evidence="ECO:0000259|Pfam:PF00148"
SQ   SEQUENCE   519 AA;  58254 MW;  776262D4EA81DC21 CRC64;
     MPHHTFKCSE CIPERTKHAV IKGADEDLTS CLPLGYLNTI PGSISERGCA YCGAKHVIGQ
     PMKDVIHISH GPIGCTYDTW QTKRYISDNN NFQLKYTYAT DVREKHIVFG AEKLLKQNIL
     EAFKAFPDIK RMTIYQTCAT ALIGDDIDAI ASEVMDELPD VDIFVCNSPG FAGPSQSGGH
     HKINIAWVNQ KVGTVEPEIT SDYVINYVGE YNIQGDQEVM QDYFNRMGIQ ILSTFTGNGS
     YDGLRAMHRA HLNVLECARS AEYICNELRV RYGIPRLDID GFGFEPLSDS LRKIGLFFGI
     EDRAQAIIDE ETAKWKPQLD WYKERLRGKK VCLWPGGSKL WHWAHVIQEE MGLNVVSLYT
     KFGHQGDMEK GIARCGEGAL AIDDPNELEG LEALEMLKPD IILTGKRPGE VAKKVRVPYL
     NAHAYHNGPY KGYEGWVRFA RDIYNAIYSP IFQLSALDIS KDPIPTDQGF LTPQMISDPA
     LPAEVRSSTV LTPYRGAYDT ISALREKTYP RFDAVPVAQ
//