Q2RTZ4 (SYE2_RHORT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Rru_A1601

Organism

Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) [Reference proteome] [HAMAP]

Taxonomic identifier

269796 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum › Rhodospirillum rubrum

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022		PRO_0000237395
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	238 – 242	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RTZ4 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 6501E735C8009F67	FASTA	468	51,709
	10 20 30 40 50 60 MTVVTRFAPS PTGFLHIGGA RTALFNWLFA RHHGGRFLLR IEDTDRVRST PEAVAAIFDG 70 80 90 100 110 120 LEWLGLDWDE EPTFQFARAA RHAEAAHELV AKGLAYRCYC TPDELTAMRE EQKAKGLPPR 130 140 150 160 170 180 YNGLWRDRDP SEAPAGVAPV IRLKAPQDGE TTITDSVQGA VTVANNQLDD MILLRSDGTP 190 200 210 220 230 240 TYMLSVVVDD HDMGVTHVIR GDDHLTNAFR QTQLYWALGW ETPVFAHIPL IHGADGAKLS 250 260 270 280 290 300 KRHGALGAEA YRDMGFLPEA LRNYLVRLGW AHGDDEVFTT EQAVEWFSLE SIGRSPSRFD 310 320 330 340 350 360 MQKLTALNGS YMRETDDDRL TALLVPRLES TLGLDIPAAG HALLRAGMAG LKERAKTLVE 370 380 390 400 410 420 LADLAAFYVR PRPLAIDAKA EKALDDEGRT ILAALIDKLD DFSPWTRDSL ENLARVMSEE 430 440 450 460 RGVKLGKVAQ PIRAALTGST VSPPIFEVME ILGPDETLGR LRDAQSHS

References

[1]

"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000230 Genomic DNA. Translation: ABC22401.1.
RefSeq	YP_426688.1. NC_007643.1.
3D structure databases
ProteinModelPortal	Q2RTZ4.
ModBase	Search...
Protein-protein interaction databases
STRING	269796.Rru_A1601.
Proteomic databases
PRIDE	Q2RTZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC22401; ABC22401; Rru_A1601.
GeneID	3835018.
KEGG	rru:Rru_A1601.
PATRIC	23326842. VBIRhoRub82919_1675.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	RYRDFKG.
ProtClustDB	PRK01406.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_RHORT

Accession

Primary (citable) accession number: Q2RTZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program