Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD(P) transhydrogenase subunit alpha part 1

Gene

pntAA

Organism
Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.By similarity

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234NAD; via carbonyl oxygen3 Publications1
Binding sitei247NAD3 Publications1
Binding sitei266NAD; via carbonyl oxygen3 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 129NAD3 Publications3
Nucleotide bindingi132 – 135NAD3 Publications4
Nucleotide bindingi180 – 182NAD3 Publications3
Nucleotide bindingi202 – 204NAD3 Publications3

GO - Molecular functioni

  • NAD(P)+ transhydrogenase (AB-specific) activity Source: UniProtKB
  • NAD(P)+ transhydrogenase (B-specific) activity Source: CACAO
  • NAD+ binding Source: UniProtKB
  • NAD binding Source: UniProtKB
  • NADH binding Source: UniProtKB
  • protein dimerization activity Source: UniProtKB

GO - Biological processi

  • NADPH regeneration Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi1.6.1.2 5420

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P) transhydrogenase subunit alpha part 1 (EC:1.6.1.2)
Alternative name(s):
Nicotinamide nucleotide transhydrogenase subunit alpha 1
Proton-translocating transhydrogenase component 1
Pyridine nucleotide transhydrogenase subunit alpha 1
dI
Gene namesi
Name:pntAA
Synonyms:nntA1
Ordered Locus Names:Rru_A2183
OrganismiRhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Taxonomic identifieri269796 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum
Proteomesi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi127R → A or M: No effect on interaction with PntB, but hydride transfer inhibited. Much weaker binding to NADH. 1 Publication1
Mutagenesisi132Q → N: No effect on interaction with PntB, but hydride transfer strongly inhibited. No effect on NADH binding affinity on its own, but differences in NADH binding properties in complex with PntB. 1 Publication1
Mutagenesisi135D → N: No effect on interaction with PntB, but hydride transfer strongly inhibited. No effect on the binding affinity to NADH. 1 Publication1
Mutagenesisi138S → A: No effect on interaction with PntB, but hydride transfer strongly inhibited. No effect on the binding affinity to NADH. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002316631 – 384NAD(P) transhydrogenase subunit alpha part 1Add BLAST384

Interactioni

Subunit structurei

Heterotrimer of two alpha chains and a beta (PntB) chain; in Rhodospirillum, the alpha chain is made of two subunits (PntAA and PntAB) and forms a dimer.5 Publications

GO - Molecular functioni

  • protein dimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi269796.Rru_A2183

Structurei

Secondary structure

1384
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Beta strandi10 – 12Combined sources3
Helixi20 – 28Combined sources9
Beta strandi32 – 36Combined sources5
Turni37 – 40Combined sources4
Helixi41 – 43Combined sources3
Helixi47 – 52Combined sources6
Beta strandi56 – 60Combined sources5
Helixi61 – 65Combined sources5
Beta strandi69 – 75Combined sources7
Helixi80 – 82Combined sources3
Helixi86 – 89Combined sources4
Beta strandi95 – 99Combined sources5
Helixi102 – 104Combined sources3
Helixi106 – 114Combined sources9
Beta strandi118 – 121Combined sources4
Helixi122 – 124Combined sources3
Helixi129 – 134Combined sources6
Helixi136 – 155Combined sources20
Beta strandi163 – 165Combined sources3
Beta strandi168 – 170Combined sources3
Beta strandi174 – 178Combined sources5
Helixi182 – 193Combined sources12
Beta strandi197 – 201Combined sources5
Helixi208 – 213Combined sources6
Turni223 – 228Combined sources6
Beta strandi233 – 235Combined sources3
Helixi247 – 255Combined sources9
Beta strandi259 – 263Combined sources5
Beta strandi268 – 270Combined sources3
Helixi278 – 281Combined sources4
Beta strandi289 – 292Combined sources4
Helixi295 – 297Combined sources3
Beta strandi309 – 312Combined sources4
Beta strandi315 – 318Combined sources4
Helixi323 – 327Combined sources5
Helixi328 – 343Combined sources16
Helixi344 – 346Combined sources3
Turni349 – 352Combined sources4
Beta strandi358 – 360Combined sources3
Helixi361 – 366Combined sources6
Beta strandi367 – 370Combined sources4
Beta strandi371 – 374Combined sources4
Beta strandi379 – 381Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F8GX-ray2.00A/B/C/D1-384[»]
1HZZX-ray2.50A/B1-384[»]
1L7DX-ray1.81A/B/C/D1-384[»]
1L7EX-ray1.90A/B/C/D1-384[»]
1NM5X-ray2.40A/B1-384[»]
1PTJX-ray2.61A/B1-381[»]
1U28X-ray2.30A/B1-384[»]
1U2DX-ray3.00A/B1-384[»]
1U2GX-ray2.20A/B1-384[»]
1XLTX-ray3.10A/B/D/E/G/H1-384[»]
2FR8X-ray2.60A/B1-384[»]
2FRDX-ray3.20A/B1-384[»]
2FSVX-ray2.30A/B1-384[»]
2OO5X-ray2.60A/B1-384[»]
2OORX-ray2.32A/B1-384[»]
ProteinModelPortaliQ2RSB2
SMRiQ2RSB2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2RSB2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 136RQD loop; involved in interaction with PntBAdd BLAST11

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Phylogenomic databases

eggNOGiENOG4108IIE Bacteria
COG3288 LUCA
HOGENOMiHOG000022121
KOiK00324
OMAiAKVFIMG
OrthoDBiPOG091H0397

Family and domain databases

InterProiView protein in InterPro
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RSB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAIPKERR PGEDRVAISP EVVKKLVGLG FEVIVEQGAG VGASITDDAL
60 70 80 90 100
TAAGATIAST AAQALSQADV VWKVQRPMTA EEGTDEVALI KEGAVLMCHL
110 120 130 140 150
GALTNRPVVE ALTKRKITAY AMELMPRISR AQSMDILSSQ SNLAGYRAVI
160 170 180 190 200
DGAYEFARAF PMMMTAAGTV PPARVLVFGV GVAGLQAIAT AKRLGAVVMA
210 220 230 240 250
TDVRAATKEQ VESLGGKFIT VDDEAMKTAE TAGGYAKEMG EEFRKKQAEA
260 270 280 290 300
VLKELVKTDI AITTALIPGK PAPVLITEEM VTKMKPGSVI IDLAVEAGGN
310 320 330 340 350
CPLSEPGKIV VKHGVKIVGH TNVPSRVAAD ASPLFAKNLL NFLTPHVDKD
360 370 380
TKTLVMKLED ETVSGTCVTR DGAIVHPALT GQGA
Length:384
Mass (Da):40,277
Last modified:January 24, 2006 - v1
Checksum:iB886A640CE2BFA12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05294 Genomic DNA Translation: AAA62493.1
CP000230 Genomic DNA Translation: ABC22983.1
RefSeqiWP_011390032.1, NC_007643.1
YP_427270.1, NC_007643.1

Genome annotation databases

EnsemblBacteriaiABC22983; ABC22983; Rru_A2183
GeneIDi3835610
KEGGirru:Rru_A2183
PATRICifig|269796.9.peg.2277

Similar proteinsi

Entry informationi

Entry nameiPNTAA_RHORT
AccessioniPrimary (citable) accession number: Q2RSB2
Secondary accession number(s): Q60164
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 24, 2006
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health