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Protein

Thiazole synthase

Gene

thiG

Organism
Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei132Schiff-base intermediate with DXPUniRule annotation1
Binding sitei193DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:Rru_A2432
OrganismiRhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Taxonomic identifieri269796 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum
Proteomesi
  • UP000001929 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002363621 – 289Thiazole synthaseAdd BLAST289

Proteomic databases

PRIDEiQ2RRL3

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi269796.Rru_A2432

Structurei

3D structure databases

ProteinModelPortaliQ2RRL3
SMRiQ2RRL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 220DXP bindingUniRule annotation2
Regioni241 – 242DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
COG2104 LUCA
HOGENOMiHOG000248049
KOiK03149
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.10.20.30, 1 hit
3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR012675 Beta-grasp_dom_sf
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RRL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTTYKETA LDEGDQLEIV HFIGGGDRSD PQPADDPFTV AGRVFSSRLL
60 70 80 90 100
VGTGKYRDFE ETARAIEASG AEIVTVAVRR VNVSDPSQPM LVDYVDPAKY
110 120 130 140 150
TYLPNTAGCF TADEAVRTLR LAREAGGWSL VKLEVLGDQR TLYPNMAETF
160 170 180 190 200
LAAEALIRDG FEVMVYCADD PIAAKRLEDM GCVAIMPLGS LIGSGLGIIN
210 220 230 240 250
PYTIRLIKES VSVPVLVDAG VGTASDAAVA MELGCDGVLM NTAIAQAKDP
260 270 280
VRMARAMRLA IEAGRLSYQA GRMPRKLYAD PSSPLAGLI
Length:289
Mass (Da):30,952
Last modified:May 30, 2006 - v2
Checksum:i9275B30D6BFF9503
GO

Sequence cautioni

The sequence ABC23232 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000230 Genomic DNA Translation: ABC23232.1 Different initiation.
RefSeqiYP_427519.2, NC_007643.1

Genome annotation databases

EnsemblBacteriaiABC23232; ABC23232; Rru_A2432
GeneIDi3835866
KEGGirru:Rru_A2432
PATRICifig|269796.9.peg.2535

Similar proteinsi

Entry informationi

Entry nameiTHIG_RHORT
AccessioniPrimary (citable) accession number: Q2RRL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: May 23, 2018
This is version 76 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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