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Protein

Thiazole synthase

Gene

thiG

Organism
Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei132 – 1321Schiff-base intermediate with DXPUniRule annotation
Binding sitei193 – 1931DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciRRUB269796:GCN1-2474-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:Rru_A2432
OrganismiRhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Taxonomic identifieri269796 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum
Proteomesi
  • UP000001929 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Thiazole synthasePRO_0000236362Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi269796.Rru_A2432.

Structurei

3D structure databases

ProteinModelPortaliQ2RRL3.
SMRiQ2RRL3. Positions 37-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni219 – 2202DXP bindingUniRule annotation
Regioni241 – 2422DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
COG2104. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OrthoDBiEOG6KMBD9.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR012675. Beta-grasp_dom.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2RRL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTTYKETA LDEGDQLEIV HFIGGGDRSD PQPADDPFTV AGRVFSSRLL
60 70 80 90 100
VGTGKYRDFE ETARAIEASG AEIVTVAVRR VNVSDPSQPM LVDYVDPAKY
110 120 130 140 150
TYLPNTAGCF TADEAVRTLR LAREAGGWSL VKLEVLGDQR TLYPNMAETF
160 170 180 190 200
LAAEALIRDG FEVMVYCADD PIAAKRLEDM GCVAIMPLGS LIGSGLGIIN
210 220 230 240 250
PYTIRLIKES VSVPVLVDAG VGTASDAAVA MELGCDGVLM NTAIAQAKDP
260 270 280
VRMARAMRLA IEAGRLSYQA GRMPRKLYAD PSSPLAGLI
Length:289
Mass (Da):30,952
Last modified:May 30, 2006 - v2
Checksum:i9275B30D6BFF9503
GO

Sequence cautioni

The sequence ABC23232.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000230 Genomic DNA. Translation: ABC23232.1. Different initiation.
RefSeqiYP_427519.2. NC_007643.1.

Genome annotation databases

EnsemblBacteriaiABC23232; ABC23232; Rru_A2432.
GeneIDi3835866.
KEGGirru:Rru_A2432.
PATRICi23328592. VBIRhoRub82919_2535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000230 Genomic DNA. Translation: ABC23232.1. Different initiation.
RefSeqiYP_427519.2. NC_007643.1.

3D structure databases

ProteinModelPortaliQ2RRL3.
SMRiQ2RRL3. Positions 37-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269796.Rru_A2432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC23232; ABC23232; Rru_A2432.
GeneIDi3835866.
KEGGirru:Rru_A2432.
PATRICi23328592. VBIRhoRub82919_2535.

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
COG2104. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OrthoDBiEOG6KMBD9.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciRRUB269796:GCN1-2474-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR012675. Beta-grasp_dom.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.
    , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1.

Entry informationi

Entry nameiTHIG_RHORT
AccessioniPrimary (citable) accession number: Q2RRL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: July 6, 2016
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.