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Q2RNG2

- GLND_RHORT

UniProt

Q2RNG2 - GLND_RHORT

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, Rru_A3539
Organism
Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciRRUB269796:GCN1-3602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Rru_A3539
OrganismiRhodospirillum rubrum (strain ATCC 11170 / NCIB 8255)
Taxonomic identifieri269796 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum
ProteomesiUP000001929: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000231691Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269796.Rru_A3539.

Structurei

3D structure databases

ProteinModelPortaliQ2RNG2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini491 – 58595HD
Add
BLAST
Domaini728 – 80982ACT 1
Add
BLAST
Domaini840 – 91576ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 372372UridylyltransferaseUniRule annotation
Add
BLAST
Regioni373 – 727355Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2RNG2-1 [UniParc]FASTAAdd to Basket

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MTIPRIRQPR AVIDRKALTV VLEDLAATVT DNRERRARLL AVLKGALGDG    50
RAEVRRRFLE EKGTGAAVFA ENSHLMDQII RLLFDFTTTH VYPRANRTIG 100
EQMTVLAVGG YGRGEMSPQS DVDLLFLLPY KATPLHEQVV EYMLYTLWDM 150
GLKVGHATRS IEECIRQARG DLTIRTAMLE TRYLWGDRAL YGQLKTKFWT 200
GVVTGTGPDF VEAKLAERDE RHLRMGDSRY VLEPNIKDGK GGLRDLHTLL 250
WIARYIYGVS DMRELVELGV LSADAATKFG RARAFLWTVR CHLHYLADRP 300
EERLTFDVQP AIAARMGYTD RNSGRGVERF MKHYFLMAKT VGDLTRIFCA 350
VLEDQQKRRP ILSIATLLMR KRNLGDFVLD GGRLAVAGRG AFREHPLQLI 400
SLFKVAHDHG LDIHPDTLRL VTEHLPTVTL LRNDAKANAL FMEILTSRKD 450
PELALRQLSE SGVLARFIPD FGRVTAQMQF DMYHVYTTDE HTIRAIGLLH 500
RLETGALRDR MPAAADAVHK VQSRRALYLA VLLHDIAKGR GGDHSILGAE 550
VAMRLGPRLG MSEEETETVA WLVRHHLDMS RTAFKRDLDD IKTILDFTGL 600
VQSVERLHLL LALTTVDILA VGPAVWNNWK SSLLRELYTH SKDVLTSGFQ 650
AEARDKRVAH KREELAAALA DWPQASRERY LDLHYPAYWL TFDSATHLRH 700
ARMLRRARDA GLTVAVEVLP DPERAVSEVL VATDDHPGLF SKIAGAMALA 750
GVNILDAKIT TMSDGGALDI FTVQTLEGHA IEKEERIARL AKTVRDVLTG 800
DLPLEKALRR QPPRLPERTR HLTVPPRVIV DNQASKTHTV IEINGRDRPG 850
FLYAVTRALT DVAVQISSAR VSTYGERVVD SFYVKDVFGM KIVHRAKLAQ 900
IREALEAAIT QTVPRKVEEG AEQGAEKADA GEIVAA 936
Length:936
Mass (Da):105,094
Last modified:January 24, 2006 - v1
Checksum:i6F952FC64FC6A76C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000230 Genomic DNA. Translation: ABC24333.1.
RefSeqiWP_011391286.1. NC_007643.1.
YP_428620.1. NC_007643.1.

Genome annotation databases

EnsemblBacteriaiABC24333; ABC24333; Rru_A3539.
GeneIDi3836994.
KEGGirru:Rru_A3539.
PATRICi23330876. VBIRhoRub82919_3656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000230 Genomic DNA. Translation: ABC24333.1 .
RefSeqi WP_011391286.1. NC_007643.1.
YP_428620.1. NC_007643.1.

3D structure databases

ProteinModelPortali Q2RNG2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 269796.Rru_A3539.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC24333 ; ABC24333 ; Rru_A3539 .
GeneIDi 3836994.
KEGGi rru:Rru_A3539.
PATRICi 23330876. VBIRhoRub82919_3656.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci RRUB269796:GCN1-3602-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.
    , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S.1.

Entry informationi

Entry nameiGLND_RHORT
AccessioniPrimary (citable) accession number: Q2RNG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 24, 2006
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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