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Q2RN70 (SYL_RHORT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Rru_A3631
OrganismRhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) [Reference proteome] [HAMAP]
Taxonomic identifier269796 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334805

Regions

Motif44 – 5411"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2RN70 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 9D92FEB9305F579A

FASTA86295,530
        10         20         30         40         50         60 
MASEDRYNVK ETEAKWQTAW AEGGCFTARE DRTRPKYYVL EMFPYPSGRI HMGHVRNYTL 

        70         80         90        100        110        120 
GDVVARFKRA QGFNVLHPMG WDAFGLPAEN AAIQRGVHPA TWTRQNIAIM REQFKPMGLS 

       130        140        150        160        170        180 
IDWSREVSTC EPAYYRHEQK MFLDFLKAGL AYRRESWVNW DPVEHTVLAN EQVIDGKGWR 

       190        200        210        220        230        240 
SGAPVERRKL SQWFLRITRY AEDLLAAIGD LDRWPDKVRL MQTNWIGRSE GARVRFGLVG 

       250        260        270        280        290        300 
RDQALEVYTT RPDTLFGASF CAISANHPLA AEIAATNPDL AAFIAECGRM GTSEVEIETA 

       310        320        330        340        350        360 
EKKGFDTGLK VRHPFDPAWE LPVYVANFVL MEYGTGAIFG CPAHDQRDMD FARKYGLPVR 

       370        380        390        400        410        420 
AVVAPAGVDA EAFAKDLEAS DTAFSEDGVA IASGFLDGLP VSEAKARAIA HIAELGAGEG 

       430        440        450        460        470        480 
VVQFRLRDWG VSRQRYWGCP IPIIHCDDCG PVPVPEDQLP VLLPEDVTFD KPGNPLDHHP 

       490        500        510        520        530        540 
TWKHVACPQC GKPARRETDT FDTFFESSWY FARFCAPHDT DRAFEREAVD YWLPVDQYVG 

       550        560        570        580        590        600 
GVEHAVLHLL YSRFFTRALR DCGYLGVSEP FTGLFTQGMI CHETYRDTDG AWLSPDQIDK 

       610        620        630        640        650        660 
AADGTATLLS DGSPVSVGRS EKMSKSKMNT VDPTAILESY GADAARLFML SDSPPDRDMD 

       670        680        690        700        710        720 
WTDSGIEGAW RYIGRLWRMA LQPQIDPGAV GAPLPADLGP GAAALVRHVH KAVAGVTDDL 

       730        740        750        760        770        780 
EKFRFNAAVA RLRELTNAIA ALDGKEAGAG AVYRFALETA ARLAAPMIPH IAEEMWSHLG 

       790        800        810        820        830        840 
RDGLLAETPW PTWDPLMLID DQVTIAVQVN GKMRGTVDLP KDAKREDAEA AALALPTVLA 

       850        860 
QLAGAAPRKV IVVPNRIINV VV 

« Hide

References

[1]"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C.
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000230 Genomic DNA. Translation: ABC24425.1.
RefSeqYP_428712.1. NC_007643.1.

3D structure databases

ProteinModelPortalQ2RN70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269796.Rru_A3631.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC24425; ABC24425; Rru_A3631.
GeneID3837087.
KEGGrru:Rru_A3631.
PATRIC23331070. VBIRhoRub82919_3752.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRRUB269796:GCN1-3695-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHORT
AccessionPrimary (citable) accession number: Q2RN70
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries