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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Moorella thermoacetica (strain ATCC 39073 / JCM 9320)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.
L-glutamine + H2O = L-glutamate + NH3.

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Nucleophile1
Binding sitei105L-glutamine1
Active sitei170Charge relay system1
Active sitei172Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Protein family/group databases

MEROPSiC26.A32.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxT (EC:4.3.3.6)
Alternative name(s):
Pdx2
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2)
Gene namesi
Name:pdxT
Ordered Locus Names:Moth_0010
OrganismiMoorella thermoacetica (strain ATCC 39073 / JCM 9320)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002558341 – 188Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST188

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.

Protein-protein interaction databases

STRINGi264732.Moth_0010.

Structurei

3D structure databases

ProteinModelPortaliQ2RMI9.
SMRiQ2RMI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 48L-glutamine binding3
Regioni134 – 135L-glutamine binding2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX. Bacteria.
COG0311. LUCA.
HOGENOMiHOG000039949.
KOiK08681.
OMAiVFIRAPI.
OrthoDBiPOG091H084H.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
PANTHERiPTHR31559. PTHR31559. 1 hit.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2RMI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVLAMQG AFREHIQSLE ALGVQGVEIR HANQLEGIAG LIIPGGESTT
60 70 80 90 100
IGKLMVEFNL LEPVRHLAEG GLPVFGTCAG MVLLARDIIG SDQPRLGLMN
110 120 130 140 150
ARVQRNAFGR QVDSFEVDLE IPVLGEEPFH AVFIRAPYIE EIEPPAEALA
160 170 180
TFKDKIVMVR QGNLLATAFH PELTKDLRVH SYFLKMIG
Length:188
Mass (Da):20,636
Last modified:January 24, 2006 - v1
Checksum:iBF0F6DB685732B5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA. Translation: ABC18350.1.
RefSeqiWP_011391559.1. NC_007644.1.
YP_428893.1. NC_007644.1.

Genome annotation databases

EnsemblBacteriaiABC18350; ABC18350; Moth_0010.
GeneIDi3831882.
KEGGimta:Moth_0010.
PATRICifig|264732.11.peg.11.

Similar proteinsi

Entry informationi

Entry nameiPDXT_MOOTA
AccessioniPrimary (citable) accession number: Q2RMI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: November 22, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families