ID FTHS_MOOTA Reviewed; 559 AA. AC Q2RM91; DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot. DT 24-JAN-2006, sequence version 1. DT 27-MAR-2024, entry version 99. DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543}; DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543}; DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543}; DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543}; DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543}; GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; GN OrderedLocusNames=Moth_0109; OS Moorella thermoacetica (strain ATCC 39073 / JCM 9320). OC Bacteria; Bacillota; Clostridia; Moorellales; Moorellaceae; Moorella. OX NCBI_TaxID=264732; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 39073 / JCM 9320; RX PubMed=18631365; DOI=10.1111/j.1462-2920.2008.01679.x; RA Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., RA Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.; RT "The complete genome sequence of Moorella thermoacetica (f. Clostridium RT thermoaceticum)."; RL Environ. Microbiol. 10:2550-2573(2008). CC -!- CATALYTIC ACTIVITY: CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10- CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221, CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216; CC EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543}; CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. CC {ECO:0000255|HAMAP-Rule:MF_01543}. CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family. CC {ECO:0000255|HAMAP-Rule:MF_01543}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000232; ABC18448.1; -; Genomic_DNA. DR RefSeq; YP_428991.1; NC_007644.1. DR PDB; 4IOJ; X-ray; 2.20 A; A/B=1-559. DR PDB; 4IOK; X-ray; 2.50 A; A/B=1-559. DR PDB; 4IOL; X-ray; 2.56 A; A/B=1-559. DR PDB; 4IOM; X-ray; 3.00 A; A/B=1-559. DR PDBsum; 4IOJ; -. DR PDBsum; 4IOK; -. DR PDBsum; 4IOL; -. DR PDBsum; 4IOM; -. DR AlphaFoldDB; Q2RM91; -. DR SMR; Q2RM91; -. DR STRING; 264732.Moth_0109; -. DR EnsemblBacteria; ABC18448; ABC18448; Moth_0109. DR KEGG; mta:Moth_0109; -. DR PATRIC; fig|264732.11.peg.114; -. DR eggNOG; COG2759; Bacteria. DR HOGENOM; CLU_003601_3_3_9; -. DR OrthoDB; 9761733at2; -. DR UniPathway; UPA00193; -. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule. DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. DR CDD; cd00477; FTHFS; 1. DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1. DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR HAMAP; MF_01543; FTHFS; 1. DR InterPro; IPR000559; Formate_THF_ligase. DR InterPro; IPR020628; Formate_THF_ligase_CS. DR InterPro; IPR027417; P-loop_NTPase. DR Pfam; PF01268; FTHFS; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR PROSITE; PS00721; FTHFS_1; 1. DR PROSITE; PS00722; FTHFS_2; 1. PE 1: Evidence at protein level; KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding; KW One-carbon metabolism. FT CHAIN 1..559 FT /note="Formate--tetrahydrofolate ligase" FT /id="PRO_0000300530" FT BINDING 68..75 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543" FT HELIX 7..12 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 19..25 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 30..32 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 33..39 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 41..43 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 45..50 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 51..53 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 58..65 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 74..87 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 92..96 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 102..105 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 106..108 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 112..114 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 117..120 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 122..126 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 127..130 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 132..152 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 161..163 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 168..172 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 175..177 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 178..182 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 187..189 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 193..195 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 197..199 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 200..202 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 204..211 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 215..224 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 226..230 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 235..237 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 239..241 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 244..250 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 251..255 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 258..262 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 267..270 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 276..279 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 285..294 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 296..305 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 306..308 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 309..315 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 317..321 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 327..332 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 334..340 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 345..347 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 353..371 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 372..374 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 377..382 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 389..401 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 405..409 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 411..414 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 415..419 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 420..432 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 447..457 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 464..466 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 468..479 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 496..498 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 511..513 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 515..519 FT /evidence="ECO:0007829|PDB:4IOJ" FT TURN 520..523 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 524..528 FT /evidence="ECO:0007829|PDB:4IOJ" FT HELIX 543..546 FT /evidence="ECO:0007829|PDB:4IOJ" FT STRAND 555..557 FT /evidence="ECO:0007829|PDB:4IOJ" SQ SEQUENCE 559 AA; 59942 MW; D8CD2BB52FC35A4D CRC64; MSKVPSDIEI AQAAKMKPVM ELARGLGIQE DEVELYGKYK AKISLDVYRR LKDKPDGKLI LVTAITPTPA GEGKTTTSVG LTDALARLGK RVMVCLREPS LGPSFGIKGG AAGGGYAQVV PMEDINLHFT GDIHAVTYAH NLLAAMVDNH LQQGNVLNID PRTITWRRVI DLNDRALRNI VIGLGGKANG VPRETGFDIS VASEVMACLC LASDLMDLKE RFSRIVVGYT YDGKPVTAGD LEAQGSMALL MKDAIKPNLV QTLENTPAFI HGGPFANIAH GCNSIIATKT ALKLADYVVT EAGFGADLGA EKFYDVKCRY AGFKPDATVI VATVRALKMH GGVPKSDLAT ENLEALREGF ANLEKHIENI GKFGVPAVVA INAFPTDTEA ELNLLYELCA KAGAEVALSE VWAKGGEGGL ELARKVLQTL ESRPSNFHVL YNLDLSIKDK IAKIATEIYG ADGVNYTAEA DKAIQRYESL GYGNLPVVMA KTQYSFSDDM TKLGRPRNFT ITVREVRLSA GAGFIVPITG AIMTMPGLPK RPAACNIDID ADGVITGLF //