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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Moorella thermoacetica (strain ATCC 39073 / JCM 9320)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei261ATPUniRule annotation1
Binding sitei283ATP; via amide nitrogenUniRule annotation1
Binding sitei292ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 180ATPUniRule annotation2
Nucleotide bindingi204 – 205ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE264732:G1G5J-215-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:Moth_0198
OrganismiMoorella thermoacetica (strain ATCC 39073 / JCM 9320)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000742451 – 390Probable tRNA sulfurtransferaseAdd BLAST390

Proteomic databases

PRIDEiQ2RM06

Interactioni

Protein-protein interaction databases

STRINGi264732.Moth_0198

Structurei

3D structure databases

ProteinModelPortaliQ2RM06
SMRiQ2RM06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 161THUMPUniRule annotationAdd BLAST104

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8I Bacteria
COG0301 LUCA
HOGENOMiHOG000227470
KOiK03151
OMAiKLFPEIM
OrthoDBiPOG091H03I8

Family and domain databases

CDDicd01712 ThiI, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00021 ThiI, 1 hit
InterProiView protein in InterPro
IPR014729 Rossmann-like_a/b/a_fold
IPR020536 ThiI_AANH
IPR004114 THUMP_dom
IPR003720 tRNA_STrfase
PfamiView protein in Pfam
PF02568 ThiI, 1 hit
PF02926 THUMP, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
TIGRFAMsiTIGR00342 TIGR00342, 1 hit
PROSITEiView protein in PROSITE
PS51165 THUMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RM06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTSLLVRYG EISLKGNNRP YFEDKLLANM RRALAGLPPR RMRKTFGRVF
60 70 80 90 100
VELHDDLEAV ARRLQRVFGI VSMSPVATAP LELEAIKKAA LAVLKDSPGS
110 120 130 140 150
TFKVQAQRPN KRFPLTSPEV NQELGAYLLT HSQGQRVDVH HPDRVIHVEI
160 170 180 190 200
RDEGAYIYSR IIPGPGGLPV GVTGRGLLLI SGGIDSPVAG YMGMKRGLEL
210 220 230 240 250
TALHFHSFPF TSERSKEKVI DLCRVLAGYS GPLRLVVAPF TNIQKAIRQN
260 270 280 290 300
CPQEFYVTIM RRMMFRIARA VAAKEEAPAI LTGESLGQVA SQTLQSMAVI
310 320 330 340 350
NKVVDLPVLR PLVAWDKSEI IEVARRIGTY DISIRPYEDC CTLFVPKHPA
360 370 380 390
TKPPLARVEA AEKNLAVVEL VAECLENLEI LTVEPEADVV
Length:390
Mass (Da):43,237
Last modified:January 24, 2006 - v1
Checksum:i035FB245B58B5538
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC18533.1
RefSeqiWP_011391742.1, NC_007644.1
YP_429076.1, NC_007644.1

Genome annotation databases

EnsemblBacteriaiABC18533; ABC18533; Moth_0198
GeneIDi3832271
KEGGimta:Moth_0198
PATRICifig|264732.11.peg.210

Similar proteinsi

Entry informationi

Entry nameiTHII_MOOTA
AccessioniPrimary (citable) accession number: Q2RM06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 24, 2006
Last modified: February 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health