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Q2RM06

- THII_MOOTA

UniProt

Q2RM06 - THII_MOOTA

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Protein
Probable tRNA sulfurtransferase
Gene
thiI, Moth_0198
Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei261 – 2611ATP By similarity
Binding sitei283 – 2831ATP; via amide nitrogen By similarity
Binding sitei292 – 2921ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1802ATP By similarity
Nucleotide bindingi204 – 2052ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA thio-modification Source: UniProtKB-HAMAP
  2. thiamine biosynthetic process Source: UniProtKB-HAMAP
  3. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciMTHE264732:GH0A-215-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferase (EC:2.8.1.4)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene namesi
Name:thiI
Ordered Locus Names:Moth_0198
OrganismiMoorella thermoacetica (strain ATCC 39073)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
ProteomesiUP000007053: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Probable tRNA sulfurtransferaseUniRule annotation
PRO_1000074245Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264732.Moth_0198.

Structurei

3D structure databases

ProteinModelPortaliQ2RM06.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 161104THUMP
Add
BLAST

Sequence similaritiesi

Belongs to the ThiI family.
Contains 1 THUMP domain.

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227470.
KOiK03151.
OMAiAQMKGPD.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2RM06-1 [UniParc]FASTAAdd to Basket

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MYTSLLVRYG EISLKGNNRP YFEDKLLANM RRALAGLPPR RMRKTFGRVF    50
VELHDDLEAV ARRLQRVFGI VSMSPVATAP LELEAIKKAA LAVLKDSPGS 100
TFKVQAQRPN KRFPLTSPEV NQELGAYLLT HSQGQRVDVH HPDRVIHVEI 150
RDEGAYIYSR IIPGPGGLPV GVTGRGLLLI SGGIDSPVAG YMGMKRGLEL 200
TALHFHSFPF TSERSKEKVI DLCRVLAGYS GPLRLVVAPF TNIQKAIRQN 250
CPQEFYVTIM RRMMFRIARA VAAKEEAPAI LTGESLGQVA SQTLQSMAVI 300
NKVVDLPVLR PLVAWDKSEI IEVARRIGTY DISIRPYEDC CTLFVPKHPA 350
TKPPLARVEA AEKNLAVVEL VAECLENLEI LTVEPEADVV 390
Length:390
Mass (Da):43,237
Last modified:January 24, 2006 - v1
Checksum:i035FB245B58B5538
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000232 Genomic DNA. Translation: ABC18533.1.
RefSeqiYP_429076.1. NC_007644.1.

Genome annotation databases

EnsemblBacteriaiABC18533; ABC18533; Moth_0198.
GeneIDi3832271.
KEGGimta:Moth_0198.
PATRICi22637419. VBIMooThe6753_0210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000232 Genomic DNA. Translation: ABC18533.1 .
RefSeqi YP_429076.1. NC_007644.1.

3D structure databases

ProteinModelPortali Q2RM06.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264732.Moth_0198.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC18533 ; ABC18533 ; Moth_0198 .
GeneIDi 3832271.
KEGGi mta:Moth_0198.
PATRICi 22637419. VBIMooThe6753_0210.

Phylogenomic databases

eggNOGi COG0301.
HOGENOMi HOG000227470.
KOi K03151.
OMAi AQMKGPD.
OrthoDBi EOG6TBHGR.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci MTHE264732:GH0A-215-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
PROSITEi PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39073.

Entry informationi

Entry nameiTHII_MOOTA
AccessioniPrimary (citable) accession number: Q2RM06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 24, 2006
Last modified: July 9, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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