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Q2RLV3 (Q2RLV3_MOOTA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204
Ordered Locus Names:Moth_0251
OrganismMoorella thermoacetica (strain ATCC 39073) [Reference proteome] [HAMAP] EMBL ABC18586.1
Taxonomic identifier264732 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Moorella group › Moorella ›

Protein attributes

Sequence length663 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204 SAAS SAAS024759

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. HAMAP-Rule MF_00204 RuleBase RU003587

Contains 1 UVR domain. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Contains 1 helicase ATP-binding domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain25 – 180156Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain429 – 595167Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain626 – 66136UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding38 – 458ATP By similarity HAMAP-Rule MF_00204
Motif91 – 11424Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
Q2RLV3 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: D63C58FC80198C85

FASTA66375,834
        10         20         30         40         50         60 
MPPFILKSDY QPRGDQPRAI AALVEGLKKG YRHQTLLGAT GTGKTYTMAQ VIQAVQRPTL 

        70         80         90        100        110        120 
VLAPNKTLAA QLCGEFKEFF PDNAVEYFVS YYDYYQPEAY VPQTDTYIEK DSSINDEIDK 

       130        140        150        160        170        180 
LRHSATAALF ERRDVIIVAS VSCIYGLGSP EDYSTLMLSL REGQEYDRDA ILRKLVDIQY 

       190        200        210        220        230        240 
SRNDYDFKRG TFRVRGDVIE IFPASFTEKA IRVEMFGDEI ERLLEIDTLT GEILGRRSHV 

       250        260        270        280        290        300 
AVFPASHYVV EEAKMERALE SIQAELEERL RELRAQGKLL EAQRLEQRTN FDLEMMREVG 

       310        320        330        340        350        360 
FCKGIENYSR HLTGRAPGEP PYTLLDYFPD DFLMMIDESH ITVPQIGGMY EGDRSRKETL 

       370        380        390        400        410        420 
VEYGFRLPSA LDNRPLTFEE FCRHINQVIY VSATPGPYEL EHSQQVVEQI IRPTGLVDPE 

       430        440        450        460        470        480 
VLVRPVKGQI DDLLAEIQKR VAKNQRVLVT TLTKRMAEDL TDYLREAGLK VRYMHSDIGA 

       490        500        510        520        530        540 
IERMEIIRDL RLGVFDVLVG INLLREGLDL PEVSLVAILD ADKEGFLRSE RSLIQTIGRA 

       550        560        570        580        590        600 
ARNAEGQVIM YADTITDSMR RAIDETNRRR QIQMEYNRRH GITPRTVSKP VREVIEATRA 

       610        620        630        640        650        660 
AEEPARYETA GEGARKKTKL SKRELKALIS QLEKEMRAAA KRLEFERAAE LRDAILELRL 


QAG

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References

[1]"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)."
Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.
Environ. Microbiol. 10:2550-2573(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39073.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000232 Genomic DNA. Translation: ABC18586.1.
RefSeqYP_429129.1. NC_007644.1.

3D structure databases

ProteinModelPortalQ2RLV3.
SMRQ2RLV3. Positions 4-655.
ModBaseSearch...

Protein-protein interaction databases

STRING264732.Moth_0251.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC18586; ABC18586; Moth_0251.
GeneID3833214.
KEGGmta:Moth_0251.
PATRIC22637533. VBIMooThe6753_0267.

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMACIYGLGI.
ProtClustDBPRK05298.

Enzyme and pathway databases

BioCycMTHE264732:GH0A-343-MONOMER.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ2RLV3_MOOTA
AccessionPrimary (citable) accession number: Q2RLV3
Entry history
Integrated into UniProtKB/TrEMBL: January 24, 2006
Last sequence update: January 24, 2006
Last modified: May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info