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Q2RKZ1 (SYL_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Moth_0568
OrganismMoorella thermoacetica (strain ATCC 39073) [Reference proteome] [HAMAP]
Taxonomic identifier264732 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 829829Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334772

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif582 – 5865"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5851ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2RKZ1 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 61B06E2164BF6103

FASTA82994,798
        10         20         30         40         50         60 
MEARYNFKEI EPKWQRRWEA SDLYRVTEDP DKPKFYCLEM FPYPSGNLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARVKRMRG YNVLHPMGWD AFGLPAENAA IHRGIPPAEW TWSNIANMRR QLHAMGISYD 

       130        140        150        160        170        180 
WDREVATCHP NYYRWTQWLF LQMYKHGLAY RKKAAVNWCP SCATVLANEQ VVDGACERCH 

       190        200        210        220        230        240 
TPVMRKDLEQ WFFRITDYAD RLLEDLKKLP GWPDKVKIMQ ENWIGKSSGA EVVFRVEGSG 

       250        260        270        280        290        300 
EEIPVFTTRP DTLYGVTYLV LAPEHPLVEK LTAGTPYEGP VEEFVQAARY LSALDRTATE 

       310        320        330        340        350        360 
KEKEGLFTGA YAINPVNNER VPIWIANYVL MEYGTGAVMG VPAHDQRDFE FARKYDLPVK 

       370        380        390        400        410        420 
VVIRPASSEL PAGELAAAYV EDGIMVNSGP FNGLPNREGI QKVTEYLESI GRGRARVNYR 

       430        440        450        460        470        480 
LRDWLISRQR YWGAPIPMIY CDQCGIVPVP EEDLPVILPE GVEFRPTGES PLTYCPEFVN 

       490        500        510        520        530        540 
TTCPRCGGPA RRETDTMDTF VCSSWYFLRY TSPHSQDRAF ERDKVDYWMN VDQYIGGVEH 

       550        560        570        580        590        600 
AILHLMYARF FTKALHDFGL VGVEEPFQNL LTQGMVLKDG SKMSKSKGNI VSPEEIINRY 

       610        620        630        640        650        660 
GADTARLFIL FAAPPERDLE WSDQGVEGCY RFLNRVWRLV GSYADAVRRA GGSLEIRSHA 

       670        680        690        700        710        720 
DRELWRLLHA TIKKVTEDVE ERFNFNTAIS AIMELVNGCY RYQDTVPEEE QNLVLMGEVL 

       730        740        750        760        770        780 
RKLVTLLAPF APHITEELWQ GLGGQESVHR ESWPQYDPEA LVEEEITLVI QINGKVKDRM 

       790        800        810        820 
QVPAGLAREK IEELVLNRDK VAALLAGQQV VKVIVVPDKL VNVVARRAS 

« Hide

References

[1]"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)."
Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.
Environ. Microbiol. 10:2550-2573(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39073.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000232 Genomic DNA. Translation: ABC18898.1.
RefSeqYP_429441.1. NC_007644.1.

3D structure databases

ProteinModelPortalQ2RKZ1.
SMRQ2RKZ1. Positions 4-761.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264732.Moth_0568.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC18898; ABC18898; Moth_0568.
GeneID3832481.
KEGGmta:Moth_0568.
PATRIC22638241. VBIMooThe6753_0611.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMTHE264732:GH0A-600-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MOOTA
AccessionPrimary (citable) accession number: Q2RKZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries