Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [ATP] 1

Gene

pckA1

Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551SubstrateUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation
Metal bindingi196 – 1961ManganeseUniRule annotation
Binding sitei196 – 1961ATPUniRule annotation
Binding sitei196 – 1961SubstrateUniRule annotation
Metal bindingi215 – 2151Manganese; via tele nitrogenUniRule annotation
Binding sitei215 – 2151ATPUniRule annotation
Metal bindingi252 – 2521ManganeseUniRule annotation
Binding sitei280 – 2801ATPUniRule annotation
Binding sitei317 – 3171ATPUniRule annotation
Binding sitei317 – 3171SubstrateUniRule annotation
Binding sitei442 – 4421ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2399ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE264732:GH0A-669-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP] 1UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCK 1UniRule annotation
Short name:
PEP carboxykinase 1UniRule annotation
Short name:
PEPCK 1UniRule annotation
Gene namesi
Name:pckA1UniRule annotation
Ordered Locus Names:Moth_0633
OrganismiMoorella thermoacetica (strain ATCC 39073)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Phosphoenolpyruvate carboxykinase [ATP] 1PRO_0000236928Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264732.Moth_0633.

Structurei

3D structure databases

ProteinModelPortaliQ2RKS7.
SMRiQ2RKS7. Positions 6-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2RKS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTYGLERL GIINPGTIYR NLPMARLVEI ALARGEGLLA SNGALSVNTG
60 70 80 90 100
KYTGRSPHDR YIVDTPAVHD SISWGAVNQP VSEATFERLY SRLTAYLQGK
110 120 130 140 150
DLFVFDGFVG ADPAYRMPIR IVNEYAWQNL FVHQLFIRPT AEELAGHEPR
160 170 180 190 200
FTVICAPGFK AIPEEDGTRS EAFIILNFDR RLVIIGGTSY AGEMKKSIFT
210 220 230 240 250
VMNYLLPEQG VCPMHCSANM GPAGDTALFF GLSGTGKTTL SADPERYLIG
260 270 280 290 300
DDEHGWSDKG IFNFEGGCYA KCIKLSAEHE PQIWNAIRFG SVLENVMVDP
310 320 330 340 350
DCRIIDYDSD ALTENTRAAY PVDFIPNAVI PGVGGHPQTV VFLTADAFGV
360 370 380 390 400
MPPIAKLTRE QAMYYFLSGY TSKLAGTERG VTEPKATFST CFGAPFLPRS
410 420 430 440 450
PMVYANLLGE RIARHNASVY LVNTGWTGGP YGTGRRMSLP YTRAMVRAAL
460 470 480 490 500
NGELDKVEFT PDPVFGFLVP KACPGVPAEI LNPRNTWAET EKYDAMARKL
510 520
ASLFRENFAK FKDVPVSIQE AGVVG
Length:525
Mass (Da):57,646
Last modified:January 24, 2006 - v1
Checksum:i3544D0360ED1BFE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA. Translation: ABC18962.1.
RefSeqiWP_011392169.1. NC_007644.1.
YP_429505.1. NC_007644.1.

Genome annotation databases

EnsemblBacteriaiABC18962; ABC18962; Moth_0633.
GeneIDi3832531.
KEGGimta:Moth_0633.
PATRICi22638383. VBIMooThe6753_0680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA. Translation: ABC18962.1.
RefSeqiWP_011392169.1. NC_007644.1.
YP_429505.1. NC_007644.1.

3D structure databases

ProteinModelPortaliQ2RKS7.
SMRiQ2RKS7. Positions 6-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264732.Moth_0633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC18962; ABC18962; Moth_0633.
GeneIDi3832531.
KEGGimta:Moth_0633.
PATRICi22638383. VBIMooThe6753_0680.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciMTHE264732:GH0A-669-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39073.

Entry informationi

Entry nameiPCKA1_MOOTA
AccessioniPrimary (citable) accession number: Q2RKS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 24, 2006
Last modified: December 9, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.