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Q2RKL6

- PNPH_MOOTA

UniProt

Q2RKL6 - PNPH_MOOTA

Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

Moth_0705

Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91PhosphateUniRule annotation
    Sitei164 – 1641Important for substrate specificityUniRule annotation
    Binding sitei182 – 1821Substrate; via amide nitrogenUniRule annotation
    Binding sitei183 – 1831PhosphateUniRule annotation
    Sitei218 – 2181Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
    3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. purine ribonucleoside salvage Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMTHE264732:GH0A-741-MONOMER.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
    Alternative name(s):
    Purine nucleoside phosphorylaseUniRule annotation
    Short name:
    PNPUniRule annotation
    Gene namesi
    Ordered Locus Names:Moth_0705
    OrganismiMoorella thermoacetica (strain ATCC 39073)
    Taxonomic identifieri264732 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
    ProteomesiUP000007053: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Probable 6-oxopurine nucleoside phosphorylasePRO_0000415082Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi264732.Moth_0705.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2RKL6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 502Phosphate bindingUniRule annotation
    Regioni206 – 2083Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000228987.
    KOiK00772.
    OMAiHVVGMTA.
    OrthoDBiEOG6KHFXC.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2RKL6-1 [UniParc]FASTAAdd to Basket

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    MRIAIIGGSG VYDPGILTNI HEERVETPYG AAVLKVGTYH GEEIGFMPRH    50
    GDKHTVPPHK VNYRANIWAL KMLKVERVLA TAAVGSTNPE FRPGDFVIVN 100
    DFLDFTKTRT YTFFEGGETG VVHTDFTTPY CPELGQVLVE TAARLGIKAH 150
    AGGVYACTEG PRFETPAEIR MIRQLGGDLV GMTNVPEVVL AHEVGLCYGL 200
    IAMVTNMAAG ISSTPLSHEE VLEIMDQNGK NLRDLIMQAI PGIPRQRNCR 250
    CSLAAGKIEV 260
    Length:260
    Mass (Da):28,319
    Last modified:January 24, 2006 - v1
    Checksum:i336D6524DA87688F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000232 Genomic DNA. Translation: ABC19023.1.
    RefSeqiYP_429566.1. NC_007644.1.

    Genome annotation databases

    EnsemblBacteriaiABC19023; ABC19023; Moth_0705.
    GeneIDi3832706.
    KEGGimta:Moth_0705.
    PATRICi22638531. VBIMooThe6753_0754.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000232 Genomic DNA. Translation: ABC19023.1 .
    RefSeqi YP_429566.1. NC_007644.1.

    3D structure databases

    ProteinModelPortali Q2RKL6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264732.Moth_0705.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC19023 ; ABC19023 ; Moth_0705 .
    GeneIDi 3832706.
    KEGGi mta:Moth_0705.
    PATRICi 22638531. VBIMooThe6753_0754.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000228987.
    KOi K00772.
    OMAi HVVGMTA.
    OrthoDBi EOG6KHFXC.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .
    BioCyci MTHE264732:GH0A-741-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39073.

    Entry informationi

    Entry nameiPNPH_MOOTA
    AccessioniPrimary (citable) accession number: Q2RKL6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3