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Q2RKL6 (PNPH_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 6-oxopurine nucleoside phosphorylase

EC=2.4.2.1
Alternative name(s):
Purine nucleoside phosphorylase
Short name=PNP
Gene names
Ordered Locus Names:Moth_0705
OrganismMoorella thermoacetica (strain ATCC 39073) [Reference proteome] [HAMAP]
Taxonomic identifier264732 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity. HAMAP-Rule MF_01963

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260Probable 6-oxopurine nucleoside phosphorylase HAMAP-Rule MF_01963
PRO_0000415082

Regions

Region49 – 502Phosphate binding By similarity
Region206 – 2083Substrate binding By similarity

Sites

Binding site91Phosphate By similarity
Binding site1821Substrate; via amide nitrogen By similarity
Binding site1831Phosphate By similarity
Site1641Important for substrate specificity By similarity
Site2181Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2RKL6 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 336D6524DA87688F

FASTA26028,319
        10         20         30         40         50         60 
MRIAIIGGSG VYDPGILTNI HEERVETPYG AAVLKVGTYH GEEIGFMPRH GDKHTVPPHK 

        70         80         90        100        110        120 
VNYRANIWAL KMLKVERVLA TAAVGSTNPE FRPGDFVIVN DFLDFTKTRT YTFFEGGETG 

       130        140        150        160        170        180 
VVHTDFTTPY CPELGQVLVE TAARLGIKAH AGGVYACTEG PRFETPAEIR MIRQLGGDLV 

       190        200        210        220        230        240 
GMTNVPEVVL AHEVGLCYGL IAMVTNMAAG ISSTPLSHEE VLEIMDQNGK NLRDLIMQAI 

       250        260 
PGIPRQRNCR CSLAAGKIEV 

« Hide

References

[1]"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)."
Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.
Environ. Microbiol. 10:2550-2573(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39073.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000232 Genomic DNA. Translation: ABC19023.1.
RefSeqYP_429566.1. NC_007644.1.

3D structure databases

ProteinModelPortalQ2RKL6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264732.Moth_0705.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC19023; ABC19023; Moth_0705.
GeneID3832706.
KEGGmta:Moth_0705.
PATRIC22638531. VBIMooThe6753_0754.

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000228987.
KOK00772.
OMAHVVGMTA.
OrthoDBEOG6KHFXC.

Enzyme and pathway databases

BioCycMTHE264732:GH0A-741-MONOMER.
UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01694. MTAP. 1 hit.
ProtoNetSearch...

Entry information

Entry namePNPH_MOOTA
AccessionPrimary (citable) accession number: Q2RKL6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways