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Q2RKL6

- PNPH_MOOTA

UniProt

Q2RKL6 - PNPH_MOOTA

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Protein
Probable 6-oxopurine nucleoside phosphorylase
Gene
Moth_0705
Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Phosphate By similarity
Sitei164 – 1641Important for substrate specificity By similarity
Binding sitei182 – 1821Substrate; via amide nitrogen By similarity
Binding sitei183 – 1831Phosphate By similarity
Sitei218 – 2181Important for substrate specificity By similarity

GO - Molecular functioni

  1. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro
  2. phosphorylase activity Source: InterPro
  3. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMTHE264732:GH0A-741-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylase (EC:2.4.2.1)
Alternative name(s):
Purine nucleoside phosphorylase
Short name:
PNP
Gene namesi
Ordered Locus Names:Moth_0705
OrganismiMoorella thermoacetica (strain ATCC 39073)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
ProteomesiUP000007053: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation
PRO_0000415082Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi264732.Moth_0705.

Structurei

3D structure databases

ProteinModelPortaliQ2RKL6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 502Phosphate binding By similarity
Regioni206 – 2083Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiHVVGMTA.
OrthoDBiEOG6KHFXC.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2RKL6-1 [UniParc]FASTAAdd to Basket

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MRIAIIGGSG VYDPGILTNI HEERVETPYG AAVLKVGTYH GEEIGFMPRH    50
GDKHTVPPHK VNYRANIWAL KMLKVERVLA TAAVGSTNPE FRPGDFVIVN 100
DFLDFTKTRT YTFFEGGETG VVHTDFTTPY CPELGQVLVE TAARLGIKAH 150
AGGVYACTEG PRFETPAEIR MIRQLGGDLV GMTNVPEVVL AHEVGLCYGL 200
IAMVTNMAAG ISSTPLSHEE VLEIMDQNGK NLRDLIMQAI PGIPRQRNCR 250
CSLAAGKIEV 260
Length:260
Mass (Da):28,319
Last modified:January 24, 2006 - v1
Checksum:i336D6524DA87688F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000232 Genomic DNA. Translation: ABC19023.1.
RefSeqiYP_429566.1. NC_007644.1.

Genome annotation databases

EnsemblBacteriaiABC19023; ABC19023; Moth_0705.
GeneIDi3832706.
KEGGimta:Moth_0705.
PATRICi22638531. VBIMooThe6753_0754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000232 Genomic DNA. Translation: ABC19023.1 .
RefSeqi YP_429566.1. NC_007644.1.

3D structure databases

ProteinModelPortali Q2RKL6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 264732.Moth_0705.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC19023 ; ABC19023 ; Moth_0705 .
GeneIDi 3832706.
KEGGi mta:Moth_0705.
PATRICi 22638531. VBIMooThe6753_0754.

Phylogenomic databases

eggNOGi COG0005.
HOGENOMi HOG000228987.
KOi K00772.
OMAi HVVGMTA.
OrthoDBi EOG6KHFXC.

Enzyme and pathway databases

UniPathwayi UPA00606 .
BioCyci MTHE264732:GH0A-741-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39073.

Entry informationi

Entry nameiPNPH_MOOTA
AccessioniPrimary (citable) accession number: Q2RKL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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