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Q2RK59

- SYI_MOOTA

UniProt

Q2RK59 - SYI_MOOTA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei555 – 5551Aminoacyl-adenylateUniRule annotation
    Binding sitei599 – 5991ATPUniRule annotation
    Metal bindingi896 – 8961ZincUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation
    Metal bindingi916 – 9161ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMTHE264732:GH0A-899-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Moth_0863
    OrganismiMoorella thermoacetica (strain ATCC 39073)
    Taxonomic identifieri264732 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
    ProteomesiUP000007053: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Isoleucine--tRNA ligasePRO_1000022092Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi264732.Moth_0863.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2RK59.
    SMRiQ2RK59. Positions 1-926.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi596 – 6005"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2RK59-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSKTLNLP RTDFPMRANL PQREPEILKF WEENDIYGKV QEANRGKPKF    50
    ILHDGPPYAN GHLHLGHTLN KILKDIIIKY HSMNGYDAPY VPGWDTHGLP 100
    IEQQAIKNLG LNRHAVDVVE FRNRCRDYAL KYVNIQREEF KRLGVRGDWE 150
    HPYLTLEPEY EAIQIGIFGE MAKKGYIYKG LKPVYWCTDC ETALAEAEVE 200
    YGEERSPSIY VKFPVADARG LFEPRGSSIV IWTTTPWTLP ANVAIALHPE 250
    FKYVLIQVGE ERLLMAAELV RPVLELLGVK DYQVVATFTG SELEGVVCRN 300
    PLMDRDSVVI LGEHVTLEQG TGCVHTAPGH GLEDYEVGMR YHLPVLSPLD 350
    DRGRFTEEGG QFAGLFIDEA NKAVVKELEA RGALLHFGFI KHQYPHCWRC 400
    KHPIIFRATE QWFASIEGFR QEALEAIKKV KWIPSWGEDR IYNMVADRSD 450
    WCISRQRTWG VPIPIFYCAN CGREIINDAT ISHLQELFRK HGSNVWFARE 500
    AGELVPPGLK CPECGSKEFR KETDIMDVWF DSGSSHAAVL ATRPELAWPA 550
    DLYLEGSDQH RGWFNSSLST AVATRGRAPY RQVLTHGFLV DEEGRKMSKS 600
    LGNGIDPADV IRQRGADVLR LWVASADYRR DVAASENIMR QITEAYRKIR 650
    NTCRFLLANL ADFDPGKDQV PREEMLELDR WAMNRLQRLI ARVTMAYDDY 700
    EFHVVYHTIH NFCAVDLSAV YLDIIKDRLY TWPAASKGRR SAQTVLYETI 750
    NVLVRLLTPI LAFTTEEIWR YLPGEDDRPI SVQLAGWPQV KTEFLDDELE 800
    EKWKRILQVR DVVARALERA RQEQDLGNSL NAAVHLYPDA DMYQFLKPLG 850
    DELATIMIVS RVDLHQPGEE APAGSLEAPE LPGLRVYVTG APGQKCERCW 900
    MVSETVGQDT DHPTLCRRCA TVVKEMYMNG 930
    Length:930
    Mass (Da):106,238
    Last modified:January 24, 2006 - v1
    Checksum:iF09E475EDD3423AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000232 Genomic DNA. Translation: ABC19180.1.
    RefSeqiYP_429723.1. NC_007644.1.

    Genome annotation databases

    EnsemblBacteriaiABC19180; ABC19180; Moth_0863.
    GeneIDi3831750.
    KEGGimta:Moth_0863.
    PATRICi22638876. VBIMooThe6753_0926.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000232 Genomic DNA. Translation: ABC19180.1 .
    RefSeqi YP_429723.1. NC_007644.1.

    3D structure databases

    ProteinModelPortali Q2RK59.
    SMRi Q2RK59. Positions 1-926.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264732.Moth_0863.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC19180 ; ABC19180 ; Moth_0863 .
    GeneIDi 3831750.
    KEGGi mta:Moth_0863.
    PATRICi 22638876. VBIMooThe6753_0926.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MTHE264732:GH0A-899-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39073.

    Entry informationi

    Entry nameiSYI_MOOTA
    AccessioniPrimary (citable) accession number: Q2RK59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3