Q2RJU7 (NUOA_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	Moth_0977

Organism

Moorella thermoacetica (strain ATCC 39073)

Taxonomic identifier

264732 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Moorella group › Moorella

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_5000105751
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	63 – 83	21	Helical; Potential
	Transmembrane	89 – 109	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2RJU7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: DBA120C407683CA3	FASTA	120	13,497
	10 20 30 40 50 60 MVLQQYGIIA VFLVGGAATA VAALATNWLL RPKKPPEGDK LAAYECGLKT QGPTWIQFKV 70 80 90 100 110 120 SYFLYALVFL LFDVETVFLY PWAVRFQALG LFAFAEMIVF IGILVLGLWY AWKEGALKWL

References

[1]

"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)." Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W. Environ. Microbiol. 10:2550-2573(2008) [PubMed: 18631365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39073.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000232 Genomic DNA. Translation: ABC19292.1.
RefSeq	YP_429835.1. NC_007644.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RJU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3831253.
GenomeReviews	Gene locus Moth_0977 in contig CP000232_GR.
KEGG	mta:Moth_0977.
NMPDR	fig|264732.9.peg.957.
PATRIC	22639128. VBIMooThe6753_1051.
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HBG605540.
OMA	GERELTY.
PhylomeDB	Q2RJU7.
ProtClustDB	CLSK943542.
Enzyme and pathway databases
BioCyc	MTHE264732:MOTH_0977-MONOMER.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_MOOTA

Accession

Primary (citable) accession number: Q2RJU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program