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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Moorella thermoacetica (strain ATCC 39073 / JCM 9320)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei254ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE264732:G1G5J-1174-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Moth_1132
OrganismiMoorella thermoacetica (strain ATCC 39073 / JCM 9320)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002373701 – 490Glutamate--tRNA ligaseAdd BLAST490

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264732.Moth_1132

Structurei

3D structure databases

ProteinModelPortaliQ2RJE3
SMRiQ2RJE3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi251 – 255"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RJE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVRVRFAP SPTGSLHIGG ARTALFNWLF ARHHNGTFVL RIDDTDTERS
60 70 80 90 100
TEASYKEILA AMGWLGLDWD EGPEKGGQFG PYLQSQRLEL YRREAARLLN
110 120 130 140 150
EGKAYLCYCT VEELAERRRQ AQAEGRPPMY DRRCRYLTPA DRTRLEQEGR
160 170 180 190 200
QPVIRLAVPE TGTTVVKDLI RGDVAFENAT IDDFIIFKSN GMPTYNFATV
210 220 230 240 250
IDDHLMQISH IIRAEEHLSN TPKQILVYQA LAYELPAFAH VPMILAPDRS
260 270 280 290 300
KLSKRHGATS VEEYRDEGYL PEAIINYLAL LGWSPEGEEE IIPLEKIIEQ
310 320 330 340 350
FSLERVSKNA AIYDTKKLTW INGHYLREGN LDRITRLALP FLQAKGLLPD
360 370 380 390 400
PLPEKDYNYV RSVIAAVRDR VKTLAEVADA ASYFFTDVTN YEEKGIRKHF
410 420 430 440 450
TRPGAAALLD EARERLATLP EFNAQAAEEA YRSLAEGKGI STGQLFHPTR
460 470 480 490
LAISGRTMGP GLFEIMELLG RETVLARLDR AARWIRENLA
Length:490
Mass (Da):55,581
Last modified:January 24, 2006 - v1
Checksum:iA60780CF76C6095D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC19446.1
RefSeqiWP_011392646.1, NC_007644.1
YP_429989.1, NC_007644.1

Genome annotation databases

EnsemblBacteriaiABC19446; ABC19446; Moth_1132
GeneIDi3833230
KEGGimta:Moth_1132
PATRICifig|264732.11.peg.1214

Similar proteinsi

Entry informationi

Entry nameiSYE_MOOTA
AccessioniPrimary (citable) accession number: Q2RJE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 24, 2006
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health