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Protein

DNA polymerase IV

Gene

dinB

Organism
Moorella thermoacetica (strain ATCC 39073 / JCM 9320)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12MagnesiumUniRule annotation1
Sitei17Substrate discriminationUniRule annotation1
Metal bindingi109MagnesiumUniRule annotation1
Active sitei110UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMTHE264732:G1G5J-1279-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:Moth_1229
OrganismiMoorella thermoacetica (strain ATCC 39073 / JCM 9320)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001371431 – 400DNA polymerase IVAdd BLAST400

Proteomic databases

PRIDEiQ2RJ46

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264732.Moth_1229

Structurei

3D structure databases

ProteinModelPortaliQ2RJ46
SMRiQ2RJ46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 191UmuCUniRule annotationAdd BLAST184

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000082707
KOiK02346
OMAiGVWIRIG
OrthoDBiPOG091H014I

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RJ46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSDCSILL CDANSFFASV HQALDPGLRG RPVIVAGREA TRHGIVLAAS
60 70 80 90 100
YEAKLGYGIK TGMTVREARG LCPHGVFIPP RHDLYIEFST RILRIMRDFT
110 120 130 140 150
PLVEPFSIDE AWLDVRGCRD LHGSPLTVAR RLKQRIREEV GITTSVGLGP
160 170 180 190 200
SKLLAKMAAE MQKPDGLTVL DYADVPGKMW PLPVRELFGI GPRMEAHLAK
210 220 230 240 250
LGIHTIGELA GFPVEVLIKR FGVVGRILHQ CARGIDYSPV DPHSLDTVKS
260 270 280 290 300
IGHQITLPRD YRGYEEIEVV LLELAELVAR RVRLGGYLGR TVAISLKDPE
310 320 330 340 350
FHWLGRSRTL PHYTDTAGDI YAAARHLLHR HWPEWRAVRL VGVSLAGLVP
360 370 380 390 400
ATVRQEDLFG RVERQARLDR ACDQLKNRYG ERVIHRAVSL TGAGVLYGGS
Length:400
Mass (Da):44,411
Last modified:January 24, 2006 - v1
Checksum:iEDA64B03008D5680
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC19543.1
RefSeqiWP_011392743.1, NC_007644.1
YP_430086.1, NC_007644.1

Genome annotation databases

EnsemblBacteriaiABC19543; ABC19543; Moth_1229
GeneIDi3833170
KEGGimta:Moth_1229
PATRICifig|264732.11.peg.1319

Similar proteinsi

Entry informationi

Entry nameiDPO4_MOOTA
AccessioniPrimary (citable) accession number: Q2RJ46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 24, 2006
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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