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Q2RHT5 (RUVC_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Moth_1702
OrganismMoorella thermoacetica (strain ATCC 39073) [Reference proteome] [HAMAP]
Taxonomic identifier264732 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella

Protein attributes

Sequence length167 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 167167Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000002776

Sites

Metal binding71Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1401Magnesium By similarity
Metal binding1431Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2RHT5 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 33EE825C12229C92

FASTA16718,047
        10         20         30         40         50         60 
MLILGIDPGT AIVGYGLVEA RAGQTRALVY DCIRTPAGED PCRRLATIYA GIRELIERLQ 

        70         80         90        100        110        120 
PETMAVEELF FNKNSKTALA VGQARGVILL AAAHTGLPVA EYTPLEVKQA VAGFGRAPKE 

       130        140        150        160 
QVQRMVQALL GLEEKPRPDD VADALAVAVC HASFAPWRQR EKEVAGR 

« Hide

References

[1]"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)."
Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.
Environ. Microbiol. 10:2550-2573(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39073.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000232 Genomic DNA. Translation: ABC20004.1.
RefSeqYP_430547.1. NC_007644.1.

3D structure databases

ProteinModelPortalQ2RHT5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264732.Moth_1702.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC20004; ABC20004; Moth_1702.
GeneID3833151.
KEGGmta:Moth_1702.
PATRIC22640730. VBIMooThe6753_1843.

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012182.
KOK01159.
OMARQVQEMV.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycMTHE264732:GH0A-1761-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_MOOTA
AccessionPrimary (citable) accession number: Q2RHT5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families