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Q2RHM6

- LIPA_MOOTA

UniProt

Q2RHM6 - LIPA_MOOTA

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Protein
Lipoyl synthase
Gene
lipA, Moth_1762
Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi48 – 481Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi69 – 691Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi76 – 761Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMTHE264732:GH0A-1824-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Moth_1762
OrganismiMoorella thermoacetica (strain ATCC 39073)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
ProteomesiUP000007053: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Lipoyl synthaseUniRule annotation
PRO_0000325277Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264732.Moth_1762.

Structurei

3D structure databases

ProteinModelPortaliQ2RHM6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2RHM6-1 [UniParc]FASTAAdd to Basket

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MVTSPSGTGT RRMPPWLHKR LPASGDIEAT RRLLADLHLN TVCQSARCPN    50
QGECFASRTA TFMILGNTCT RNCRFCAVEH GQPEAIDEDE PRRVAEAARR 100
LGLKHVVVTS VTRDDLPDGG AGHFAATITA LRQALPGAYI EVLTPDFRGS 150
RAALAAVARA RPDIFNHNVE TVPRLYPDVR PQADYRRSLD VLKQMKELDA 200
TIYTKSGLMV GVGESREEVL AVMADLRAVN CDILTIGQYL RPSPRHLEIK 250
EYVPPETFTW YAQKGREMGF LYVAAGPYVR SSYHAAEFSV KIAGVK 296
Length:296
Mass (Da):32,738
Last modified:January 24, 2006 - v1
Checksum:iE95F3CE54F3E9142
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000232 Genomic DNA. Translation: ABC20063.1.
RefSeqiYP_430606.1. NC_007644.1.

Genome annotation databases

EnsemblBacteriaiABC20063; ABC20063; Moth_1762.
GeneIDi3831054.
KEGGimta:Moth_1762.
PATRICi22640872. VBIMooThe6753_1913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000232 Genomic DNA. Translation: ABC20063.1 .
RefSeqi YP_430606.1. NC_007644.1.

3D structure databases

ProteinModelPortali Q2RHM6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 264732.Moth_1762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC20063 ; ABC20063 ; Moth_1762 .
GeneIDi 3831054.
KEGGi mta:Moth_1762.
PATRICi 22640872. VBIMooThe6753_1913.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci MTHE264732:GH0A-1824-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39073.

Entry informationi

Entry nameiLIPA_MOOTA
AccessioniPrimary (citable) accession number: Q2RHM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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