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Protein

4-hydroxy-2-oxovalerate aldolase

Gene

Moth_1775

Organism
Moorella thermoacetica (strain ATCC 39073)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds.UniRule annotation

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerUniRule annotation
Metal bindingi15 – 151ManganeseUniRule annotation
Active sitei18 – 181Proton acceptorUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Metal bindingi195 – 1951Manganese; via tele nitrogenUniRule annotation
Binding sitei195 – 1951SubstrateUniRule annotation
Metal bindingi197 – 1971Manganese; via tele nitrogenUniRule annotation
Binding sitei286 – 2861SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMTHE264732:GH0A-1837-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolaseUniRule annotation (EC:4.1.3.39UniRule annotation)
Short name:
HOAUniRule annotation
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolaseUniRule annotation
4-hydroxy-2-oxopentanoate aldolaseUniRule annotation
Gene namesi
Ordered Locus Names:Moth_1775
OrganismiMoorella thermoacetica (strain ATCC 39073)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3383384-hydroxy-2-oxovalerate aldolasePRO_0000387843Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264732.Moth_1775.

Structurei

3D structure databases

ProteinModelPortaliQ2RHL3.
SMRiQ2RHL3. Positions 2-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 152Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
KOiK01666.
OMAiWAVAGEN.
OrthoDBiEOG6C2WC0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2RHL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARSIHIVD TTLRDGSHAV SHQFTAEQIA AIAGGLDAAG VEYIEVSHGD
60 70 80 90 100
GLAGSSYNYG WAALGDEEML KAASAAIKKG KLTVLLLPGI GTVEDLKMAA
110 120 130 140 150
DCGAKVVRVA THVTEADIGE QHIGMAKKLG MMAVGFLMMC HMAPPEKVVE
160 170 180 190 200
QAKLFESYGA DYINIADSAG AMLPEDVKAR VGAVVEAVKV PVGFHAHNNL
210 220 230 240 250
TMATANALAA VEAGATFLDG ACRGLGAGAG NAQTEALVGV LDKLGYRTGV
260 270 280 290 300
DFYKVMDVAE DIVEPIMHRP QVVRNAPLML GYAGVYSSFL LHTYRAAEKF
310 320 330
NLDPRDILVE LGRRRMVGGQ EDMIVDVAYQ LAQKRGGN
Length:338
Mass (Da):35,714
Last modified:January 24, 2006 - v1
Checksum:iFB415F72F92C8B3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA. Translation: ABC20076.1.
RefSeqiWP_011393276.1. NC_007644.1.
YP_430619.1. NC_007644.1.

Genome annotation databases

EnsemblBacteriaiABC20076; ABC20076; Moth_1775.
GeneIDi3832441.
KEGGimta:Moth_1775.
PATRICi22640898. VBIMooThe6753_1926.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA. Translation: ABC20076.1.
RefSeqiWP_011393276.1. NC_007644.1.
YP_430619.1. NC_007644.1.

3D structure databases

ProteinModelPortaliQ2RHL3.
SMRiQ2RHL3. Positions 2-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264732.Moth_1775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC20076; ABC20076; Moth_1775.
GeneIDi3832441.
KEGGimta:Moth_1775.
PATRICi22640898. VBIMooThe6753_1926.

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
KOiK01666.
OMAiWAVAGEN.
OrthoDBiEOG6C2WC0.

Enzyme and pathway databases

BioCyciMTHE264732:GH0A-1837-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39073.

Entry informationi

Entry nameiHOA_MOOTA
AccessioniPrimary (citable) accession number: Q2RHL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: January 24, 2006
Last modified: November 11, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.